Diverse Bacteria Affiliated with the Genera Microvirga, Phyllobacterium, and Bradyrhizobium Nodulate Lupinus micranthus Growing in Soils of Northern Tunisia

Msaddak, Abdelhakim and Durán, David and Rejili, Mokhtar and Mars, Mohamed and Ruiz Argüeso, Tomas-Andres and Imperial Ródenas, Juan and Palacios Alberti, Jose Manuel and Rey Navarro, Luis (2017). Diverse Bacteria Affiliated with the Genera Microvirga, Phyllobacterium, and Bradyrhizobium Nodulate Lupinus micranthus Growing in Soils of Northern Tunisia. "Applied And Environmental Microbiology", v. 83 (n. 6); pp. 1-13. ISSN 0099-2240. https://doi.org/10.1128/AEM.02820-16.

Description

Title: Diverse Bacteria Affiliated with the Genera Microvirga, Phyllobacterium, and Bradyrhizobium Nodulate Lupinus micranthus Growing in Soils of Northern Tunisia
Author/s:
  • Msaddak, Abdelhakim
  • Durán, David
  • Rejili, Mokhtar
  • Mars, Mohamed
  • Ruiz Argüeso, Tomas-Andres
  • Imperial Ródenas, Juan
  • Palacios Alberti, Jose Manuel
  • Rey Navarro, Luis
Item Type: Article
Título de Revista/Publicación: Applied And Environmental Microbiology
Date: March 2017
ISSN: 0099-2240
Volume: 83
Subjects:
Freetext Keywords: Bradyrhizobium; Lupinus micranthus; Microvirga; Phyllobacterium; nodulation
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The genetic diversity of bacterial populations nodulating Lupinus micranthus in five geographical sites from northern Tunisia was examined. Phylogenetic analyses of 50 isolates based on partial sequences of recA and gyrB grouped strains into seven clusters, five of which belong to the genus Bradyrhizobium (28 isolates), one to Phyllobacterium (2 isolates), and one, remarkably, to Microvirga (20 isolates). The largest Bradyrhizobium cluster (17 isolates) grouped with the B. lupini species, and the other five clusters were close to different recently defined Bradyrhizobium species. Isolates close to Microvirga were obtained from nodules of plants from four of the five sites sampled. We carried out an in-depth phylogenetic study with representatives of the seven clusters using sequences from housekeeping genes (rrs, recA, glnII, gyrB, and dnaK) and obtained consistent results. A phylogeny based on the sequence of the symbiotic gene nodC identified four groups, three formed by Bradyrhizobium isolates and one by the Microvirga and Phyllobacterium isolates. Symbiotic behaviors of the representative strains were tested, and some congruence between symbiovars and symbiotic performance was observed. These data indicate a remarkable diversity of L. micranthus root nodule symbionts in northern Tunisia, including strains from the Bradyrhizobiaceae, Methylobacteriaceae, and Phyllobacteriaceae families, in contrast with those of the rhizobial populations nodulating lupines in the Old World, including L. micranthus from other Mediterranean areas, which are nodulated mostly by Bradyrhizobium strains.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO2013-43040UnspecifiedUnspecifiedUnspecified
Government of SpainCGL2011-26932UnspecifiedUnspecifiedUnspecified
Government of SpainCSD2009-00006UnspecifiedUnspecifiedUnspecified
Universidad Politécnica de MadridAL16-PID-06UnspecifiedUnspecifiedUnspecified

More information

Item ID: 50768
DC Identifier: http://oa.upm.es/50768/
OAI Identifier: oai:oa.upm.es:50768
DOI: 10.1128/AEM.02820-16
Official URL: http://aem.asm.org/content/83/6/e02820-16.full
Deposited by: Memoria Investigacion
Deposited on: 24 Jul 2018 08:53
Last Modified: 01 Apr 2019 12:18
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