Diverse Bacteria Affiliated with the Genera Microvirga, Phyllobacterium, and Bradyrhizobium Nodulate Lupinus micranthus Growing in Soils of Northern Tunisia

Msaddak, Abdelhakim; Durán, David; Rejili, Mokhtar; Mars, Mohamed; Ruiz Argüeso, Tomas-Andres; Imperial Ródenas, Juan; Palacios Alberti, Jose Manuel y Rey Navarro, Luis (2017). Diverse Bacteria Affiliated with the Genera Microvirga, Phyllobacterium, and Bradyrhizobium Nodulate Lupinus micranthus Growing in Soils of Northern Tunisia. "Applied And Environmental Microbiology", v. 83 (n. 6); pp. 1-13. ISSN 0099-2240. https://doi.org/10.1128/AEM.02820-16.

Descripción

Título: Diverse Bacteria Affiliated with the Genera Microvirga, Phyllobacterium, and Bradyrhizobium Nodulate Lupinus micranthus Growing in Soils of Northern Tunisia
Autor/es:
  • Msaddak, Abdelhakim
  • Durán, David
  • Rejili, Mokhtar
  • Mars, Mohamed
  • Ruiz Argüeso, Tomas-Andres
  • Imperial Ródenas, Juan
  • Palacios Alberti, Jose Manuel
  • Rey Navarro, Luis
Tipo de Documento: Artículo
Título de Revista/Publicación: Applied And Environmental Microbiology
Fecha: Marzo 2017
Volumen: 83
Materias:
Palabras Clave Informales: Bradyrhizobium; Lupinus micranthus; Microvirga; Phyllobacterium; nodulation
Escuela: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Departamento: Biotecnología - Biología Vegetal
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The genetic diversity of bacterial populations nodulating Lupinus micranthus in five geographical sites from northern Tunisia was examined. Phylogenetic analyses of 50 isolates based on partial sequences of recA and gyrB grouped strains into seven clusters, five of which belong to the genus Bradyrhizobium (28 isolates), one to Phyllobacterium (2 isolates), and one, remarkably, to Microvirga (20 isolates). The largest Bradyrhizobium cluster (17 isolates) grouped with the B. lupini species, and the other five clusters were close to different recently defined Bradyrhizobium species. Isolates close to Microvirga were obtained from nodules of plants from four of the five sites sampled. We carried out an in-depth phylogenetic study with representatives of the seven clusters using sequences from housekeeping genes (rrs, recA, glnII, gyrB, and dnaK) and obtained consistent results. A phylogeny based on the sequence of the symbiotic gene nodC identified four groups, three formed by Bradyrhizobium isolates and one by the Microvirga and Phyllobacterium isolates. Symbiotic behaviors of the representative strains were tested, and some congruence between symbiovars and symbiotic performance was observed. These data indicate a remarkable diversity of L. micranthus root nodule symbionts in northern Tunisia, including strains from the Bradyrhizobiaceae, Methylobacteriaceae, and Phyllobacteriaceae families, in contrast with those of the rhizobial populations nodulating lupines in the Old World, including L. micranthus from other Mediterranean areas, which are nodulated mostly by Bradyrhizobium strains.

Más información

ID de Registro: 50768
Identificador DC: http://oa.upm.es/50768/
Identificador OAI: oai:oa.upm.es:50768
Identificador DOI: 10.1128/AEM.02820-16
URL Oficial: http://aem.asm.org/content/83/6/e02820-16.full
Depositado por: Memoria Investigacion
Depositado el: 24 Jul 2018 08:53
Ultima Modificación: 24 Jul 2018 08:53
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