Validation of the Hirst-Type Spore Trap for Simultaneous Monitoring of Prokaryotic and Eukaryotic Biodiversities in Urban Air Samples by Next-Generation Sequencing

Nuñez Hernández, Andrés; Amo de Paz, Guillermo; Ferencova, Zuzana; Rastrojo, Alberto; Guantes, Raúl; García, Ana M.; Alcamí, Antonio; Gutiérrez-Bustillo, A. Montserrat y Moreno Gómez, Diego Alejandro (2017). Validation of the Hirst-Type Spore Trap for Simultaneous Monitoring of Prokaryotic and Eukaryotic Biodiversities in Urban Air Samples by Next-Generation Sequencing. "Applied And Environmental Microbiology", v. 83 (n. 13); pp. 1-17. ISSN 0099-2240. https://doi.org/10 .1128/AEM.00472-17.

Descripción

Título: Validation of the Hirst-Type Spore Trap for Simultaneous Monitoring of Prokaryotic and Eukaryotic Biodiversities in Urban Air Samples by Next-Generation Sequencing
Autor/es:
  • Nuñez Hernández, Andrés
  • Amo de Paz, Guillermo
  • Ferencova, Zuzana
  • Rastrojo, Alberto
  • Guantes, Raúl
  • García, Ana M.
  • Alcamí, Antonio
  • Gutiérrez-Bustillo, A. Montserrat
  • Moreno Gómez, Diego Alejandro
Tipo de Documento: Artículo
Título de Revista/Publicación: Applied And Environmental Microbiology
Fecha: 2017
Volumen: 83
Materias:
Palabras Clave Informales: airborne biodiversity; Hirst-type spore trap; bioaerosol monitoring; methods in aerobiology; next-generation sequencing (NGS)
Escuela: E.T.S.I. Industriales (UPM)
Departamento: Física Aplicada e Ingeniería de Materiales
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Pollen, fungi, and bacteria are the main microscopic biological entities present in outdoor air, causing allergy symptoms and disease transmission and having a significant role in atmosphere dynamics. Despite their relevance, a method for monitoring simultaneously these biological particles in metropolitan environments has not yet been developed. Here, we assessed the use of the Hirst-type spore trap to characterize the global airborne biota by high-throughput DNA sequencing, selecting regions of the 16S rRNA gene and internal transcribed spacer for the taxonomic assignment. We showed that aerobiological communities are well represented by this approach. The operational taxonomic units (OTUs) of two traps working synchronically compiled >87% of the total relative abundance for bacterial diversity collected in each sampler, >89% for fungi, and >97% for pollen. We found a good correspondence between traditional characterization by microscopy and genetic identification, obtaining more-accurate taxonomic assignments and detecting a greater diversity using the latter. We also demonstrated that DNA sequencing accurately detects differences in biodiversity between samples. We concluded that high-throughput DNA sequencing applied to aerobiological samples obtained with Hirst spore traps provides reliable results and can be easily implemented for monitoring prokaryotic and eukaryotic entities present in the air of urban areas.

Proyectos asociados

TipoCódigoAcrónimoResponsableTítulo
Comunidad de MadridS2013-MAE-2874AIRBIOTADiego A. MorenoConocer y modelizar la contaminación biológica del aire urbano

Más información

ID de Registro: 50843
Identificador DC: http://oa.upm.es/50843/
Identificador OAI: oai:oa.upm.es:50843
Identificador DOI: 10 .1128/AEM.00472-17
URL Oficial: http://aem.asm.org/content/83/13.toc
Depositado por: Memoria Investigacion
Depositado el: 05 Jun 2018 18:23
Ultima Modificación: 05 Jun 2018 18:23
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