Validation of the Hirst-Type Spore Trap for Simultaneous Monitoring of Prokaryotic and Eukaryotic Biodiversities in Urban Air Samples by Next-Generation Sequencing

Nuñez Hernández, Andrés and Amo de Paz, Guillermo and Ferencova, Zuzana and Rastrojo, Alberto and Guantes, Raúl and García, Ana M. and Alcamí, Antonio and Gutiérrez-Bustillo, A. Montserrat and Moreno Gómez, Diego Alejandro (2017). Validation of the Hirst-Type Spore Trap for Simultaneous Monitoring of Prokaryotic and Eukaryotic Biodiversities in Urban Air Samples by Next-Generation Sequencing. "Applied And Environmental Microbiology", v. 83 (n. 13); pp. 1-17. ISSN 0099-2240. https://doi.org/10.1128/AEM.00472-17.

Description

Title: Validation of the Hirst-Type Spore Trap for Simultaneous Monitoring of Prokaryotic and Eukaryotic Biodiversities in Urban Air Samples by Next-Generation Sequencing
Author/s:
  • Nuñez Hernández, Andrés
  • Amo de Paz, Guillermo
  • Ferencova, Zuzana
  • Rastrojo, Alberto
  • Guantes, Raúl
  • García, Ana M.
  • Alcamí, Antonio
  • Gutiérrez-Bustillo, A. Montserrat
  • Moreno Gómez, Diego Alejandro
Item Type: Article
Título de Revista/Publicación: Applied And Environmental Microbiology
Date: 2017
ISSN: 0099-2240
Volume: 83
Subjects:
Freetext Keywords: airborne biodiversity; Hirst-type spore trap; bioaerosol monitoring; methods in aerobiology; next-generation sequencing (NGS)
Faculty: E.T.S.I. Industriales (UPM)
Department: Física Aplicada e Ingeniería de Materiales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Pollen, fungi, and bacteria are the main microscopic biological entities present in outdoor air, causing allergy symptoms and disease transmission and having a significant role in atmosphere dynamics. Despite their relevance, a method for monitoring simultaneously these biological particles in metropolitan environments has not yet been developed. Here, we assessed the use of the Hirst-type spore trap to characterize the global airborne biota by high-throughput DNA sequencing, selecting regions of the 16S rRNA gene and internal transcribed spacer for the taxonomic assignment. We showed that aerobiological communities are well represented by this approach. The operational taxonomic units (OTUs) of two traps working synchronically compiled >87% of the total relative abundance for bacterial diversity collected in each sampler, >89% for fungi, and >97% for pollen. We found a good correspondence between traditional characterization by microscopy and genetic identification, obtaining more-accurate taxonomic assignments and detecting a greater diversity using the latter. We also demonstrated that DNA sequencing accurately detects differences in biodiversity between samples. We concluded that high-throughput DNA sequencing applied to aerobiological samples obtained with Hirst spore traps provides reliable results and can be easily implemented for monitoring prokaryotic and eukaryotic entities present in the air of urban areas.

Funding Projects

TypeCodeAcronymLeaderTitle
Madrid Regional GovernmentS2013-MAE-2874AIRBIOTADiego A. MorenoConocer y modelizar la contaminación biológica del aire urbano

More information

Item ID: 50843
DC Identifier: http://oa.upm.es/50843/
OAI Identifier: oai:oa.upm.es:50843
DOI: 10.1128/AEM.00472-17
Official URL: https://aem.asm.org/content/83/13/e00472-17
Deposited by: Memoria Investigacion
Deposited on: 05 Jun 2018 18:23
Last Modified: 02 Apr 2019 07:16
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