An active Mitochondrial Complex II Present in Mature Seeds Contains an Embryo-Specific Iron-Sulfur Subunit Regulated by ABA and bZIP53 and Is Involved in Germination and Seedling Establishment

Restovic, Franko and Espinoza Corral, Roberto and Gómez, Isabel and Vicente Carbajosa, Jesus and Jordana, Xavier (2017). An active Mitochondrial Complex II Present in Mature Seeds Contains an Embryo-Specific Iron-Sulfur Subunit Regulated by ABA and bZIP53 and Is Involved in Germination and Seedling Establishment. "Frontiers in Plant Science", v. 8 ; pp. 1-13. ISSN 1664-462X. https://doi.org/10.3389/fpls.2017.00277.

Description

Title: An active Mitochondrial Complex II Present in Mature Seeds Contains an Embryo-Specific Iron-Sulfur Subunit Regulated by ABA and bZIP53 and Is Involved in Germination and Seedling Establishment
Author/s:
  • Restovic, Franko
  • Espinoza Corral, Roberto
  • Gómez, Isabel
  • Vicente Carbajosa, Jesus
  • Jordana, Xavier
Item Type: Article
Título de Revista/Publicación: Frontiers in Plant Science
Date: 28 February 2017
Volume: 8
Subjects:
Freetext Keywords: mitochondrial respiratory complex; succinate dehydrogenase; iron–sulfur subunit; abscisic acid; ABI3; bZIPs; Arabidopsis seed germination; seedling establishment
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Complex II (succinate dehydrogenase) is an essential mitochondrial enzyme involved in both the tricarboxylic acid cycle and the respiratory chain. In Arabidopsis thaliana, its iron–sulfur subunit (SDH2) is encoded by three genes, one of them (SDH2.3) being specifically expressed during seed maturation in the embryo. Here we show that seed SDH2.3 expression is regulated by abscisic acid (ABA) and we define the promoter region (114 to +49) possessing all the cis-elements necessary and sufficient for high expression in seeds. This region includes between 114 and 32 three ABRE (ABAresponsive) elements and one RY-enhancer like element, and we demonstrate that these elements, although necessary, are not sufficient for seed expression, our results supporting a role for the region encoding the 5’ untranslated region (+1 to +49). The SDH2.3 promoter is activated in leaf protoplasts by heterodimers between the basic leucine zipper transcription factors bZIP53 (group S1) and bZIP10 (group C) acting through the ABRE elements, and by the B3 domain transcription factor ABA insensitive 3 (ABI3). The in vivo role of bZIP53 is further supported by decreased SDH2.3 expression in a knockdown bzip53 mutant. By using the protein synthesis inhibitor cycloheximide and sdh2 mutants we have been able to conclusively show that complex II is already present in mature embryos before imbibition, and contains mainly SDH2.3 as iron–sulfur subunit. This complex plays a role during seed germination sensu-stricto since we have previously shown that seeds lacking SDH2.3 show retarded germination and now we demonstrate that low concentrations of thenoyltrifluoroacetone, a complex II inhibitor, also delay germination. Furthermore, complex II inhibitors completely block hypocotyl elongation in the dark and seedling establishment in the light, highlighting an essential role of complex II in the acquisition of photosynthetic competence and the transition from heterotrophy to autotrophy.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO2014-53181-RUnspecifiedUnspecifiedEstrés del retículo endoplásmico en plantas: respuestas y efectos

More information

Item ID: 50953
DC Identifier: http://oa.upm.es/50953/
OAI Identifier: oai:oa.upm.es:50953
DOI: 10.3389/fpls.2017.00277
Official URL: https://www.ncbi.nlm.nih.gov/pubmed/28293251
Deposited by: Memoria Investigacion
Deposited on: 25 May 2018 08:39
Last Modified: 25 May 2018 08:39
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