Characterization of type VI secretion systems (T6SS) of endosymbionts from mimosa or lupine

Pacheco, Alba and Salinero, Álvaro and Bourebaba, Yasmina and Valle, Daniel del and Duran Wendt, David Ricardo and Boulila, Farida and Ormeño-Orrillo, E. and Martínez Romero, Esperanza and Imperial Ródenas, Juan and Ruiz Argüeso, Tomas-Andres and Palacios Alberti, Jose Manuel and Rey Navarro, Luis (2017). Characterization of type VI secretion systems (T6SS) of endosymbionts from mimosa or lupine. In: "20th International Congress on Nitrogen Fixation", 03/09/2017-07/09/2017, Granada, España. p. 1.

Description

Title: Characterization of type VI secretion systems (T6SS) of endosymbionts from mimosa or lupine
Author/s:
  • Pacheco, Alba
  • Salinero, Álvaro
  • Bourebaba, Yasmina
  • Valle, Daniel del
  • Duran Wendt, David Ricardo
  • Boulila, Farida
  • Ormeño-Orrillo, E.
  • Martínez Romero, Esperanza
  • Imperial Ródenas, Juan
  • Ruiz Argüeso, Tomas-Andres
  • Palacios Alberti, Jose Manuel
  • Rey Navarro, Luis
Item Type: Presentation at Congress or Conference (Poster)
Event Title: 20th International Congress on Nitrogen Fixation
Event Dates: 03/09/2017-07/09/2017
Event Location: Granada, España
Title of Book: Proceedings of 20th International Congress on Nitrogen Fixation
Date: September 2017
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The T6SS is a nanosyringe that injects proteins into prokaryotic or eukaryotic cells, and it is encoded in the genomes of more than 25% of Gram-negative bacteria (1). We are studying the T6SS of Rhizobium etli Mim1 and Bradyrhizobium sp. LmicA16, symbionts of Phaseolus vulgaris/Leucaena leucocephala and Lupinus micranthus/Lupinus angustifolius/Spartium junceum, respectively. R. etli Mim1 contains a T6SS gene cluster organized in two divergent operons. When the T6SS is active, Hcp, a constituent of the secretory apparatus, can be detected in the extracellular medium (2). Hcp has been immunologically detected in the supernatant of Mim1 cultures. This protein was also detected in bean nodule extracts and in cultures grown in the presence of different legumes exudates. The putative divergent promoters located between the two T6SS gene clusters were analysed by ?- gal fusions. The results showed high levels of expression of the two promoters at high OD and low values at lower ODs. Mutants affected in structural genes induced white nodules with P. vulgaris and L. leucocephala. On the other hand, mutagenesis of T6SS structural genes from LmicA16 strain produced different symbiotic phenotypes. An LmicA16 tssC mutant showed reduced levels of nitrogen fixation on L. micranthus, whereas the same mutant induced the formation of few white, non-fixing nodules on L. angustifolius and S. junceum. (1) Ho et al. (2013) Cell Host Microbe 15:9-21. (2) Wu et al. (2012) PLoS Pathog. 8:1-18 Funded by grants BIO2013-43040-P (MINECO), CGL2011-26932 (MICINN) and AL16-PID-06 (UPM).

More information

Item ID: 50990
DC Identifier: http://oa.upm.es/50990/
OAI Identifier: oai:oa.upm.es:50990
Official URL: https://www.20icnf.congresosgestac.com/es/
Deposited by: Memoria Investigacion
Deposited on: 21 Jun 2018 11:54
Last Modified: 21 Jun 2018 11:54
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