GSK-3β overexpression alters the dendritic spines of developmentally generated granule neurons in the mouse hippocampal dentate gyrus

Pallas Bazarra, Noemí and Kastanauskaite, Asta and Ávila de Grado, Jesús and Felipe Oroquieta, Javier de and Llorens Martín, María (2017). GSK-3β overexpression alters the dendritic spines of developmentally generated granule neurons in the mouse hippocampal dentate gyrus. "Frontiers in Neuroanatomy" ; pp. 11-18. ISSN 1662-5129. https://doi.org/10.3389/fnana.2017.00018.

Description

Title: GSK-3β overexpression alters the dendritic spines of developmentally generated granule neurons in the mouse hippocampal dentate gyrus
Author/s:
  • Pallas Bazarra, Noemí
  • Kastanauskaite, Asta
  • Ávila de Grado, Jesús
  • Felipe Oroquieta, Javier de
  • Llorens Martín, María
Item Type: Article
Título de Revista/Publicación: Frontiers in Neuroanatomy
Date: 2017
ISSN: 1662-5129
Subjects:
Freetext Keywords: GSK-3β; Hippocampus; Dentate gyrus; Granule neurons; Dendritic spines; Intracellular injection
Faculty: E.T.S. de Ingenieros Informáticos (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (3MB) | Preview

Abstract

The dentate gyrus (DG) plays a crucial role in hippocampal-related memory. The most abundant cellular type in the DG, namely granule neurons, are developmentally generated around postnatal day P6 in mice. Moreover, a unique feature of the DG is the occurrence of adult hippocampal neurogenesis, a process that gives rise to newborn granule neurons throughout life. Adult-born and developmentally generated granule neurons share some maturational aspects but differ in others, such as in their positioning within the granule cell layer. Adult hippocampal neurogenesis encompasses a series of plastic changes that modify the function of the hippocampal trisynaptic network. In this regard, it is known that glycogen synthase kinase 3β (GSK-3β) regulates both synaptic plasticity and memory. By using a transgenic mouse overexpressing GSK-3β in hippocampal neurons, we previously demonstrated that the overexpression of this kinase has deleterious effects on the maturation of newborn granule neurons. In the present study, we addressed the effects of GSK-3β overexpression on the morphology and number of dendritic spines of developmentally generated granule neurons. To this end, we performed intracellular injections of Lucifer Yellow in developmentally generated granule neurons of wild-type and GSK-3β-overexpressing mice and analyzed the number and morphologies of dendritic spines (namely, stubby, thin and mushroom). GSK-3β overexpression led to a general reduction in the number of dendritic spines. In addition, it caused a slight reduction in the percentage, head diameter and length of thin spines, whereas the head diameter of mushroom spines was increased.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainSAF-2014-53040-PUnspecifiedUnspecifiedUnspecified
Government of SpainSAF 2015-66603-PUnspecifiedUnspecifiedUnspecified
Government of SpainRYC-2015-17189UnspecifiedUnspecifiedUnspecified
Government of SpainCB06/05/0066UnspecifiedUnspecifiedUnspecified

More information

Item ID: 52744
DC Identifier: http://oa.upm.es/52744/
OAI Identifier: oai:oa.upm.es:52744
DOI: 10.3389/fnana.2017.00018
Official URL: https://www.frontiersin.org/articles/10.3389/fnana.2017.00018/full
Deposited by: Memoria Investigacion
Deposited on: 21 Dec 2018 11:11
Last Modified: 29 Apr 2019 14:54
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM