Mitochondrial AtTrxo1 is Transcriptionally Regulated by AtbZIP9 and AtAZF2 and Affects Seed Germination Under Saline Conditions

Ortiz Espín, Ana María and Iglesias Fernandez, Raquel and Calderón Pérez, Aingeru and Carbonero Zalduegui, Pilar and Sevilla, Francisca and Jimenez, Ana (2017). Mitochondrial AtTrxo1 is Transcriptionally Regulated by AtbZIP9 and AtAZF2 and Affects Seed Germination Under Saline Conditions. "Journal of Experimental Botany", v. 68 (n. 5); pp. 1025-1038. ISSN 0022-0957. https://doi.org/10.1093/jxb/erx012.

Description

Title: Mitochondrial AtTrxo1 is Transcriptionally Regulated by AtbZIP9 and AtAZF2 and Affects Seed Germination Under Saline Conditions
Author/s:
  • Ortiz Espín, Ana María
  • Iglesias Fernandez, Raquel
  • Calderón Pérez, Aingeru
  • Carbonero Zalduegui, Pilar
  • Sevilla, Francisca
  • Jimenez, Ana
Item Type: Article
Título de Revista/Publicación: Journal of Experimental Botany
Date: February 2017
ISSN: 0022-0957
Volume: 68
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Mitochondrial thioredoxin-o (AtTrxo1) was characterized and its expression examined in different organs of Arabidopsis thaliana. AtTrxo1 transcript levels were particularly high in dry seeds and cotyledons where they reached a maximum 36  h after imbibition with water, coinciding with 50% germination. Expression was lower in seeds germinating in 100 mM NaCl. To gain insight into the transcriptional regulation of the AtTrxo1 gene, a phylogenomic analysis was coupled with the screening of an arrayed library of Arabidopsis transcription factors in yeast. The basic leucine zipper AtbZIP9 and the zinc finger protein AZF2 were identified as putative transcriptional regulators. Transcript regulation of AtbZIP9 and AtAFZ2 during germination was compatible with the proposed role in transcriptional regulation of AtTrxo1. Transient over-expression of AtbZIP9 and AtAZF2 in Nicotiana benthamiana leaves demonstrated an acti-vation effect of AtbZIP9 and a repressor effect of AtAZF2 on AtTrxo1 promoter-driven reporter expression. Although moderate concentrations of salt delayed germination in Arabidopsis wild-type seeds, those of two different AtTrxo1 knock-out mutants germinated faster and accumulated higher H2O2 levels than the wild-type. All these data indicate that AtTrxo1 has a role in redox homeostasis during seed germination under salt conditions.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBFU/FEDER 2014-52452-PUnspecifiedUnspecifiedUnspecified
Government of SpainCSD2007-00057UnspecifiedUnspecifiedUnspecified

More information

Item ID: 53678
DC Identifier: http://oa.upm.es/53678/
OAI Identifier: oai:oa.upm.es:53678
DOI: 10.1093/jxb/erx012
Official URL: https://academic.oup.com/jxb/article/68/5/1025/2981941
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 21 Jan 2019 09:01
Last Modified: 23 Apr 2019 13:46
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