The BdGAMYB Protein from Brachypodium Distachyon Interacts with BdDOF24 and Regulates Transcription of the BdCathB Gene upon Seed Germination

González Calle, Virginia and Iglesias Fernandez, Raquel and Carbonero Zalduegui, Pilar and Barrero Sicilia, Cristina (2014). The BdGAMYB Protein from Brachypodium Distachyon Interacts with BdDOF24 and Regulates Transcription of the BdCathB Gene upon Seed Germination. "Planta", v. 240 (n. 3); pp. 539-552. ISSN 0032-0935. https://doi.org/10.1007/s00425-014-2105-3.

Description

Title: The BdGAMYB Protein from Brachypodium Distachyon Interacts with BdDOF24 and Regulates Transcription of the BdCathB Gene upon Seed Germination
Author/s:
  • González Calle, Virginia
  • Iglesias Fernandez, Raquel
  • Carbonero Zalduegui, Pilar
  • Barrero Sicilia, Cristina
Item Type: Article
Título de Revista/Publicación: Planta
Date: September 2014
ISSN: 0032-0935
Volume: 240
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

During barley seed germination, hydrolytic enzymes (α-amylases, proteases, etc.) synthesized in the aleurone layer in response to gibberellins (GA), catalyse the mobi-lization of storage reserves accumulated in the endosperm during seed maturation. In Brachypodium distachyon, the BdCathB gene that encodes a Cathepsin B-like thiol-protease, orthologous to the wheat Al21 and barley HvCathB, is highly induced in germinating seeds and its expression is regulated by transcription factors (TFs) encoded by genes BdGa-myb and BdDof24, orthologous to the barley HvGamyb and BPBF-HvDof24, respectively. Transcripts of both TF genes increase during germination and treatments with abscisic acid (ABA) or paclobutrazol (PAC, an inhibitor of GA bio-synthesis) decrease mRNA expression of BdGamyb but do not affect that of BdDof24. Besides, proteins BdDOF24 and BdGAMYB interact in yeast-2 hybrid systems and in plant nuclei, and in transient expression assays in aleurone layers BdDOF24 is a transcriptional repressor and BdGAMYB is an activator of the BdCathB promoter, as occurs with the puta-tive orthologous in barley BPBF-HvDOF24 and HvGAMYB. However, when both TFs are co-bombarded, BdDOF24 enhances the activation driven by BdGAMYB while BPBF-HvDOF24 strongly decreases the HvGAMYB-mediated activation of the BdCathB promoter. The different results obtained when BdDOF24 and BPBF-HvDOF24 interact with BdGAMYB and HvGAMYB are discussed.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBFU2009-11809UnspecifiedUnspecifiedUnspecified

More information

Item ID: 53682
DC Identifier: http://oa.upm.es/53682/
OAI Identifier: oai:oa.upm.es:53682
DOI: 10.1007/s00425-014-2105-3
Official URL: https://link.springer.com/article/10.1007/s00425-014-2105-3
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 21 Jan 2019 12:58
Last Modified: 21 Jan 2019 12:58
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