Structure, Expression Profile and Subcellular Localisation of Four Different Sucrose Synthase Genes from Barley

Barrero Sicilia, Cristina and Hernando Amado, Sara and González Melendi, Pablo and Carbonero Zalduegui, Pilar (2011). Structure, Expression Profile and Subcellular Localisation of Four Different Sucrose Synthase Genes from Barley. "Planta", v. 234 (n. 2); pp. 391-403. ISSN 0032-0935. https://doi.org/10.1007/s00425-011-1408-x.

Description

Title: Structure, Expression Profile and Subcellular Localisation of Four Different Sucrose Synthase Genes from Barley
Author/s:
  • Barrero Sicilia, Cristina
  • Hernando Amado, Sara
  • González Melendi, Pablo
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Planta
Date: January 2011
ISSN: 0032-0935
Volume: 234
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Genes encoding two new isoforms of sucrose synthase from barley, HvSs3 and HvSs4, have been characterised and their expression patterns compared with those previously described for HvSs1 and HvSs2, in different organs and during seed maturation and germination. Their response to several abiotic stimuli has also been investigated in leaves: HvSs1 is up-regulated by anoxia and HvSs3 by water deprivation while no response is observed to 150 mM NaCl treatment; HvSs1 and HvSs3 are also induced by cold temperatures. Using translational fusions and transient expression analyses, the four isozymes have been localised not only to the cytoplasm but also along several cytoplasmic tracks and at the inner side of the cell membrane; besides, HvSS1 is also associated with mitochondria, a localisation that has been predicted in silico with the TargetP and Predotar programmes. These data suggest distinct although partially overlapping roles, for the four barley sucrose synthase isoforms, in the channelling of carbon towards different metabolic pathways within the cell.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBFU2006-07258UnspecifiedPilar Carbonero ZaldueguiRedes transcripcionales en la germinación de semillas de cebada
Government of SpainBFU2009-11809UnspecifiedUnspecifiedUnspecified

More information

Item ID: 53684
DC Identifier: http://oa.upm.es/53684/
OAI Identifier: oai:oa.upm.es:53684
DOI: 10.1007/s00425-011-1408-x
Official URL: https://link.springer.com/article/10.1007/s00425-011-1408-x
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 01 Feb 2019 09:33
Last Modified: 04 Jun 2019 11:19
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