Barley BLZ2, a Seed-specific bZIP Protein That Interacts with BLZ1 in Vivo and Activates Transcription from the GCN4-like motif of B-hordein Promoters in Barley Endosperm

Oñate Sanchez, Luis and Vicente Carbajosa, Jesus and Lara, Pilar and Díaz, Isabel and Carbonero Zalduegui, Pilar (1999). Barley BLZ2, a Seed-specific bZIP Protein That Interacts with BLZ1 in Vivo and Activates Transcription from the GCN4-like motif of B-hordein Promoters in Barley Endosperm. "Journal of Biological Chemistry", v. 274 (n. 14); pp. 9175-9182. ISSN 0021-9258. https://doi.org/10.1074/jbc.274.14.9175.

Description

Title: Barley BLZ2, a Seed-specific bZIP Protein That Interacts with BLZ1 in Vivo and Activates Transcription from the GCN4-like motif of B-hordein Promoters in Barley Endosperm
Author/s:
  • Oñate Sanchez, Luis
  • Vicente Carbajosa, Jesus
  • Lara, Pilar
  • Díaz, Isabel
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Journal of Biological Chemistry
Date: April 1999
ISSN: 0021-9258
Volume: 274
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

A barley endosperm cDNA, encoding a DNA-binding protein of the bZIP class of transcription factors, BLZ2, has been characterized. The Blz2 mRNA expression is restricted to the endosperm, where it precedes that of the hordein genes. BLZ2, expressed in bacteria, binds specifically to the GCN4-like motif (GLM; 5*-GTGAGTCAT-3*) in a 43-base pair oligonucleotide derived from the promoter region of a Hor-2 gene (B1-hordein). This oligonucleotide also includes the prolamin box (PB; 5*- TGTAAAG-3*). Binding by BLZ2 is prevented when the GLM is mutated to 5*-GTGctTCtc-3* but not when mutations affect the PB. The BLZ2 protein is a potent transcriptional activator in a yeast two-hybrid system where it dimerizes with BLZ1, a barley bZIP protein encoded by the ubiquitously expressed Blz1 gene. Transient expression experiments in co-bombarded developing barley endosperms demonstrate that BLZ2 transactivates transcription from the GLM of the Hor-2 gene promoter and that this activation is also partially dependent on the presence of an intact PB. A drastic decrease in GUS activity is observed in co-bombarded barley endosperms when using as effectors equimolar mixtures of Blz2 and Blz1 in antisense constructs. These results strongly implicate the endosperm-specific BLZ2 protein from barley, either as a homodimer or as a heterodimer with BLZ1, as an important transcriptional activator of seed storage protein genes containing the GLM in their promoters.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO94-0404UnspecifiedUnspecifiedUnspecified
Government of SpainPB97-0561UnspecifiedPilar Carbonero ZaldueguiRegulación genética en semillas de cereales: caracterización molecular de factores transcripcionales tipo BZIP y DOF

More information

Item ID: 53743
DC Identifier: http://oa.upm.es/53743/
OAI Identifier: oai:oa.upm.es:53743
DOI: 10.1074/jbc.274.14.9175
Official URL: http://www.jbc.org/content/274/14/9175.long
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 25 Jan 2019 13:43
Last Modified: 25 Jan 2019 13:44
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