The TRANSPLANTA collection of Arabidopsis lines: a resource for functional analysis of transcription factors based on their conditional overexpression

Coego, Alberto and Brizuela, Esther and Castillejo, Pablo and Ruiz, Sandra and Koncz, Csaba and Pozo Benito, Juan Carlos del and Piñeiro Galvin, Manuel and Jarillo, José A. and Paz Ares, Javier and León, José and Valencia, Alfonso and Costantino, Paolo and Vittorioso, Paola and Davies, Brendan and Gilmartin, Phil and Giraudat, Jerome and Parcy, François and Reindl, Andreas and Sablowski, Robert and Coupland, George and Martin, Cathie and Angenent, Cerco C. and Baumlein, Helmut and Mock, Hans Peter and Carbonero Zalduegui, Pilar and Colombo, Lucia and Tonelli, Chiara and Engström, Peter and Dröege-Laser, Wolfgang and Gatz, Christiane and Kavanagh, Tony and Kushnir, Sergei and Zabeau, Marc and Laux, Thomas and Hordsworth, Mike and Ruberti, Ida and Ratcliff, Frank and Smeekens, Sjef and Weisshaar, Bernd and Traas, Jan (2014). The TRANSPLANTA collection of Arabidopsis lines: a resource for functional analysis of transcription factors based on their conditional overexpression. "Plant Journal", v. 77 (n. 6); pp. 944-953. ISSN 0960-7412. https://doi.org/10.1111/tpj.12443.

Description

Title: The TRANSPLANTA collection of Arabidopsis lines: a resource for functional analysis of transcription factors based on their conditional overexpression
Author/s:
  • Coego, Alberto
  • Brizuela, Esther
  • Castillejo, Pablo
  • Ruiz, Sandra
  • Koncz, Csaba
  • Pozo Benito, Juan Carlos del
  • Piñeiro Galvin, Manuel
  • Jarillo, José A.
  • Paz Ares, Javier
  • León, José
  • Valencia, Alfonso
  • Costantino, Paolo
  • Vittorioso, Paola
  • Davies, Brendan
  • Gilmartin, Phil
  • Giraudat, Jerome
  • Parcy, François
  • Reindl, Andreas
  • Sablowski, Robert
  • Coupland, George
  • Martin, Cathie
  • Angenent, Cerco C.
  • Baumlein, Helmut
  • Mock, Hans Peter
  • Carbonero Zalduegui, Pilar
  • Colombo, Lucia
  • Tonelli, Chiara
  • Engström, Peter
  • Dröege-Laser, Wolfgang
  • Gatz, Christiane
  • Kavanagh, Tony
  • Kushnir, Sergei
  • Zabeau, Marc
  • Laux, Thomas
  • Hordsworth, Mike
  • Ruberti, Ida
  • Ratcliff, Frank
  • Smeekens, Sjef
  • Weisshaar, Bernd
  • Traas, Jan
Item Type: Article
Título de Revista/Publicación: Plant Journal
Date: March 2014
ISSN: 0960-7412
Volume: 77
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Transcription factors (TFs) are key regulators of gene expression in all organisms. In eukaryotes, TFs are often represented by functionally redundant members of large gene families. Overexpression might prove a means to unveil the biological functions of redundant TFs; however, constitutive overexpression of TFs frequently causes severe developmental defects, preventing their functional characterization. Conditional overexpression strategies help to overcome this problem. Here, we report on the TRANSPLANTA collection of Arabidopsis lines, each expressing one of 949 TFs under the control of a b–estradiol-inducible promoter. Thus far, 1636 independent homozygous lines, representing an average of 2.6 lines for every TF, have been produced for the inducible expression of 634 TFs. Along with a GUS-GFP reporter, randomly selected TRANS-PLANTA lines were tested and confirmed for conditional transgene expression upon b–estradiol treatment. As a proof of concept for the exploitation of this resource, b–estradiol-induced proliferation of root hairs, dark-induced senescence, anthocyanin accumulation and dwarfism were observed in lines conditionally expressing full-length cDNAs encoding RHD6, WRKY22, MYB123/TT2 and MYB26, respectively, in agreement with previously reported phenotypes conferred by these TFs. Further screening performed with other TRANS-PLANTA lines allowed the identification of TFs involved in different plant biological processes, illustrating that the collection is a powerful resource for the functional characterization of TFs. For instance, ANAC058 and a TINY/AP2 TF were identified as modulators of ABA-mediated germination potential, and RAP2.10/DEAR4 was identified as a regulator of cell death in the hypocotyl–root transition zone. Seeds of TRANS-PLANTA lines have been deposited at the Nottingham Arabidopsis Stock Centre for further distribution.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainCONSOLIDER CSD 2007-00057TRANSPLANTAFrancisco Javier Paz AresFunción y Potencial Biotecnológico de los Factores de Transcripción en Plantas
Government of SpainBIO2011-27526UnspecifiedUnspecifiedEl óxido nítrico como modulador de la señalización mediada por ABA y giberelinas en Arabidopsis
Government of SpainBIO2010-15589UnspecifiedUnspecifiedDisección genética y molecular de mecanismos implicados en la represión de la floración

More information

Item ID: 53824
DC Identifier: http://oa.upm.es/53824/
OAI Identifier: oai:oa.upm.es:53824
DOI: 10.1111/tpj.12443
Official URL: https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.12443
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 01 Feb 2019 13:48
Last Modified: 13 Jun 2019 11:06
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