HvMCB1, a R1MYB transcription factor from barley with antagonistic regulatory functions during seed development and germination

Rubio Somoza, Ignacio and Martinez Muñoz, Manuel and Diaz Rodriguez, Isabel and Carbonero Zalduegui, Pilar (2006). HvMCB1, a R1MYB transcription factor from barley with antagonistic regulatory functions during seed development and germination. "Plant Journal", v. 45 (n. 1); pp. 17-30. ISSN 0960-7412. https://doi.org/10.1111/j.1365-313X.2005.02596.x.

Description

Title: HvMCB1, a R1MYB transcription factor from barley with antagonistic regulatory functions during seed development and germination
Author/s:
  • Rubio Somoza, Ignacio
  • Martinez Muñoz, Manuel
  • Diaz Rodriguez, Isabel
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Plant Journal
Date: January 2006
ISSN: 0960-7412
Volume: 45
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The functional analysis of hydrolase gene promoters induced by gibberellin (GA) in barley aleurone cells upon germination has identified a tripartite GA-response complex (GARC) containing a 5¢-TATCCAC-3¢ box as well as the GA-responsive element (GARE) recognized by GAMYB and the pyrimidine box interacting with the DOF transcription factors BPBF and SAD. We show here that the MCB1 gene encoding a R1MYB protein binds to the 5¢-TATCCAC-3¢ (GATA core) box in vitro and is a transcriptional repressor of a GA-induced amylase (Amy6.4) promoter in bombarded aleurone layers. Northern blot and mRNA in situ hybridization analyses showed that the MCB1 transcripts accumulate in the aleurone cells upon germination, as well as in endosperm tissues during seed development. The HvMCB1 protein expressed in bacteria binds in a specific manner to a 27-mer oligonucleotide containing the 5¢-TATCCAC-3¢ sequence, derived from the promoter region of the Amy6.4 gene. Accumulation of the MCB1 transcript diminished in response to external GA incubation in aleurone cells, and in transient expression experiments HvMCB1 repressed transcription of the Amy6.4 promoter in GA-treated aleurone layers and reversed the GAMYB-mediated activation of this amylase promoter. In contrast, during endosperm maturation HvMCB1 acted as a transcription activator of the seed-specific Itr1 gene promoter through binding to a 5¢-GATAAGATA-3¢ box.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBMC2003-06345UnspecifiedPilar Carbonero ZaldueguiGenes reguladores en semillas de cereales

More information

Item ID: 53863
DC Identifier: http://oa.upm.es/53863/
OAI Identifier: oai:oa.upm.es:53863
DOI: 10.1111/j.1365-313X.2005.02596.x
Official URL: https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-313X.2005.02596.x
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 05 Feb 2019 12:40
Last Modified: 05 Feb 2019 12:40
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