The strawberry gene Cyf1 encodes a phytocystatin with antifungal properties

Martinez Muñoz, Manuel and Abraham, Zamira and Gambardella, Marina and Echaide, Mercedes and Carbonero Zalduegui, Pilar and Diaz Rodriguez, Isabel (2005). The strawberry gene Cyf1 encodes a phytocystatin with antifungal properties. "Journal of Experimental Botany", v. 56 (n. 417); pp. 1821-1829. ISSN 0022-0957. https://doi.org/10.1093/jxb/eri172.

Description

Title: The strawberry gene Cyf1 encodes a phytocystatin with antifungal properties
Author/s:
  • Martinez Muñoz, Manuel
  • Abraham, Zamira
  • Gambardella, Marina
  • Echaide, Mercedes
  • Carbonero Zalduegui, Pilar
  • Diaz Rodriguez, Isabel
Item Type: Article
Título de Revista/Publicación: Journal of Experimental Botany
Date: July 2005
Volume: 56
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

An EST, encoding a strawberry phytocystatin (PhyCys) obtained from a developing fruit of Fragaria×ananassa cv. Elsanta has been characterized. The corresponding gene (Cyf1) had three introns interrupting its ORF that codes for a protein (FaCPI-1) of 235 amino acid residues with a putative signal peptide of 29 residues and an estimated molecular mass for the mature protein of 23.1 kDa. This protein contains, besides a C-terminal extension, several motifs conserved in all members of the PhyCys superfamily: (i) a GG and LARFAV-like motifs towards the N-terminal part of the protein; (ii) the reactive site QVVAG, and (iii) a conserved PW, downstream of the reactive site. Northern blot and in situ hybridization analyses indicated that the Cyf1 gene was expressed in fully expanded leaves, in roots and in achenes, but not in the receptacle (pseudocarp) during fruit development. The recombinant FaCPI-1 protein expressed in E. coli efficiently inhibited papain (Ki 1.9×10−9 M) and less so cathepsin H (Ki 4.7×10−7 M) and cathepsin B (Ki 3.3×10−6 M), and was a good inhibitor of the in vitro growth of phytopathogenic fungi Botrytis cinerea (EC50: 1.90 μM) and Fusarium oxysporum (EC50: 2.28 μM).

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL03-0335UnspecifiedIsabel DíazProteínas inhibidoras de hidrolasas: nuevos genes fungicidas e insecticidas
Madrid Regional Government07M/0050/2002UnspecifiedIsabel DíazEvaluación y especificidad de inhibidores de proteasas de origen vegetal frente a hongos fitopatógenos, insectos plaga y enemigos naturales

More information

Item ID: 53864
DC Identifier: http://oa.upm.es/53864/
OAI Identifier: oai:oa.upm.es:53864
DOI: 10.1093/jxb/eri172
Official URL: https://academic.oup.com/jxb/article/56/417/1821/484287
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 05 Feb 2019 11:11
Last Modified: 05 Feb 2019 11:11
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