The barley cystatin gene (Icy) is regulated by DOF transcription factors in aleurone cells upon germination

Martinez Muñoz, Manuel; Rubio Somoza, Ignacio; Fuentes, Rebeca; Lara, Pilar; Carbonero Zalduegui, Pilar y Diaz Rodriguez, Isabel (2005). The barley cystatin gene (Icy) is regulated by DOF transcription factors in aleurone cells upon germination. "Journal of Experimental Botany", v. 56 (n. 412); pp. 547-556. ISSN 0022-0957. https://doi.org/10.1093/jxb/eri033.

Descripción

Título: The barley cystatin gene (Icy) is regulated by DOF transcription factors in aleurone cells upon germination
Autor/es:
  • Martinez Muñoz, Manuel
  • Rubio Somoza, Ignacio
  • Fuentes, Rebeca
  • Lara, Pilar
  • Carbonero Zalduegui, Pilar
  • Diaz Rodriguez, Isabel
Tipo de Documento: Artículo
Título de Revista/Publicación: Journal of Experimental Botany
Fecha: Febrero 2005
Volumen: 56
Materias:
Escuela: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Departamento: Biotecnología - Biología Vegetal
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

Texto completo

[img]
Vista Previa
PDF (Document Portable Format) - Se necesita un visor de ficheros PDF, como GSview, Xpdf o Adobe Acrobat Reader
Descargar (2MB) | Vista Previa

Resumen

A genomic clone (Icy gene) encoding a barley cystatin has been characterized. The gene contains one intron interrupting its ORF that encodes a protein of 107 amino acid residues. A DNA fragment of −1058 bp upstream of the ATG translation initiation codon has been sequenced and several promoter deletions fused to the β-glucuronidase (GUS; uidA gene) reporter gene obtained. Transient expression assays in different barley tissues have indicated that a −631 bp promoter fragment was sufficient for full activity. In bombarded barley aleurone layers the GUS-driven expression by this promoter is repressed by GA3 incubation, as is the accumulation of the Icy transcripts detected. A spatial and temporal location of cystatin transcripts by in situ hybridization techniques indicates that this gene is ubiquitously expressed and its transcripts are particularly abundant in leaves and roots, and in seeds, both during development and upon germination. Two DOF transcription factors, SAD and BPBF, previously described as involved in gene expression regulation in seeds, interact specifically in vitro with oligonucleotides containing DOF binding-sites derived from the Icy gene promoter. Transient expression experiments in co-bombarded aleurone layers demonstrate that BPBF strongly represses transcription of the native Icy promoter, even when co-transfected with SAD that behaves as an activator in this in vivo system.

Proyectos asociados

TipoCódigoAcrónimoResponsableTítulo
Gobierno de EspañaAGL2003-0335Sin especificarIsabel DíazProteínas inhibidoras de hidrolasas: nuevos genes fungicidas e insecticidas
Gobierno de EspañaBIO2004-00299Sin especificarSin especificarSin especificar
Comunidad de Madrid07M/0050/2002Sin especificarIsabel DíazEvaluación y especificidad de inhibidores de proteasas de origen vegetal frente a hongos fitopatógenos, insectos plaga y enemigos naturales

Más información

ID de Registro: 53892
Identificador DC: http://oa.upm.es/53892/
Identificador OAI: oai:oa.upm.es:53892
Identificador DOI: 10.1093/jxb/eri033
URL Oficial: https://academic.oup.com/jxb/article/56/412/547/580225
Depositado por: Biblioteca ETSI Agrónomos
Depositado el: 12 Feb 2019 12:49
Ultima Modificación: 12 Feb 2019 12:49
  • InvestigaM
  • GEO_UP4
  • Open Access
  • Open Access
  • Sherpa-Romeo
    Compruebe si la revista anglosajona en la que ha publicado un artículo permite también su publicación en abierto.
  • Dulcinea
    Compruebe si la revista española en la que ha publicado un artículo permite también su publicación en abierto.
  • Recolecta
  • Observatorio I+D+i UPM
  • OpenCourseWare UPM