The barley cystatin gene (Icy) is regulated by DOF transcription factors in aleurone cells upon germination

Martinez Muñoz, Manuel and Rubio Somoza, Ignacio and Fuentes, Rebeca and Lara, Pilar and Carbonero Zalduegui, Pilar and Diaz Rodriguez, Isabel (2005). The barley cystatin gene (Icy) is regulated by DOF transcription factors in aleurone cells upon germination. "Journal of Experimental Botany", v. 56 (n. 412); pp. 547-556. ISSN 0022-0957. https://doi.org/10.1093/jxb/eri033.

Description

Title: The barley cystatin gene (Icy) is regulated by DOF transcription factors in aleurone cells upon germination
Author/s:
  • Martinez Muñoz, Manuel
  • Rubio Somoza, Ignacio
  • Fuentes, Rebeca
  • Lara, Pilar
  • Carbonero Zalduegui, Pilar
  • Diaz Rodriguez, Isabel
Item Type: Article
Título de Revista/Publicación: Journal of Experimental Botany
Date: February 2005
Volume: 56
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

A genomic clone (Icy gene) encoding a barley cystatin has been characterized. The gene contains one intron interrupting its ORF that encodes a protein of 107 amino acid residues. A DNA fragment of −1058 bp upstream of the ATG translation initiation codon has been sequenced and several promoter deletions fused to the β-glucuronidase (GUS; uidA gene) reporter gene obtained. Transient expression assays in different barley tissues have indicated that a −631 bp promoter fragment was sufficient for full activity. In bombarded barley aleurone layers the GUS-driven expression by this promoter is repressed by GA3 incubation, as is the accumulation of the Icy transcripts detected. A spatial and temporal location of cystatin transcripts by in situ hybridization techniques indicates that this gene is ubiquitously expressed and its transcripts are particularly abundant in leaves and roots, and in seeds, both during development and upon germination. Two DOF transcription factors, SAD and BPBF, previously described as involved in gene expression regulation in seeds, interact specifically in vitro with oligonucleotides containing DOF binding-sites derived from the Icy gene promoter. Transient expression experiments in co-bombarded aleurone layers demonstrate that BPBF strongly represses transcription of the native Icy promoter, even when co-transfected with SAD that behaves as an activator in this in vivo system.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2003-0335UnspecifiedIsabel DíazProteínas inhibidoras de hidrolasas: nuevos genes fungicidas e insecticidas
Government of SpainBIO2004-00299UnspecifiedUnspecifiedUnspecified
Madrid Regional Government07M/0050/2002UnspecifiedIsabel DíazEvaluación y especificidad de inhibidores de proteasas de origen vegetal frente a hongos fitopatógenos, insectos plaga y enemigos naturales

More information

Item ID: 53892
DC Identifier: http://oa.upm.es/53892/
OAI Identifier: oai:oa.upm.es:53892
DOI: 10.1093/jxb/eri033
Official URL: https://academic.oup.com/jxb/article/56/412/547/580225
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 12 Feb 2019 12:49
Last Modified: 12 Feb 2019 12:49
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