A cathepsin B‐like cysteine protease gene from Hordeum vulgare (gene CatB) induced by GA in aleurone cells is under circadian control in leaves

Martinez Muñoz, Manuel and Rubio Somoza, Ignacio and Carbonero Zalduegui, Pilar and Diaz Rodriguez, Isabel (2003). A cathepsin B‐like cysteine protease gene from Hordeum vulgare (gene CatB) induced by GA in aleurone cells is under circadian control in leaves. "Journal of Experimental Botany", v. 54 (n. 384); pp. 951-959. ISSN 0022-0957. https://doi.org/10.1093/jxb/erg099.

Description

Title: A cathepsin B‐like cysteine protease gene from Hordeum vulgare (gene CatB) induced by GA in aleurone cells is under circadian control in leaves
Author/s:
  • Martinez Muñoz, Manuel
  • Rubio Somoza, Ignacio
  • Carbonero Zalduegui, Pilar
  • Diaz Rodriguez, Isabel
Item Type: Article
Título de Revista/Publicación: Journal of Experimental Botany
Date: March 2003
ISSN: 0022-0957
Volume: 54
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

A barley cDNA clone encoding a putative cysteine protease with sequence homology to cathepsin B from mammalian cells has been characterized. This barley gene (CatB) is ubiquitously expressed, its mRNA being detected in leaves and roots, immature, mature and germinating embryos, in developing endosperms, and in aleurones upon germination, as assessed by northern blot analysis. The CatB mRNA expression in leaves increased by cold shock (6 °C), was not affected by wounding, and was under circadian control. These transcripts increased in the aleurone upon germination, whereas those for a cystatin encoding gene (Icy), that inhibits commercial cathepsin B in vitro, decreased. Gibberellin (GA) treatment of isolated barley aleurones induced and abscisic acid (ABA) repressed the steady‐state levels of CatB mRNA, while Icy expression had an opposite pattern of mRNA accumulation in aleurones treated with GA. No response to GA or ABA was detected in leaves.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBMC2000/1483UnspecifiedPilar Carbonero ZaldueguiGenes reguladores del desarrollo y la germinación de semillas de cebada y Arabidopsis
Madrid Regional Government07M/0015/2001UnspecifiedIsabel DíazEvaluación y especificidad de cistatinas de origen vegetal frente a artrópodos plaga y enemigos naturales

More information

Item ID: 53896
DC Identifier: http://oa.upm.es/53896/
OAI Identifier: oai:oa.upm.es:53896
DOI: 10.1093/jxb/erg099
Official URL: https://academic.oup.com/jxb/article/54/384/951/631175
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 11 Feb 2019 12:08
Last Modified: 11 Feb 2019 12:10
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