Transgenic expression of trypsin inhibitor CMe from barley in indica and japonica rice, confers resistance to the rice weevil Sitophilus oryzae

Alfonso Rubí, Julio and Ortego, Felix and Castañera, Pedro and Carbonero Zalduegui, Pilar and Diaz Rodriguez, Isabel (2003). Transgenic expression of trypsin inhibitor CMe from barley in indica and japonica rice, confers resistance to the rice weevil Sitophilus oryzae. "Transgenic Research", v. 12 (n. 1); pp. 23-31. ISSN 0962-8819. https://doi.org/10.1023/A:1022176207180.

Description

Title: Transgenic expression of trypsin inhibitor CMe from barley in indica and japonica rice, confers resistance to the rice weevil Sitophilus oryzae
Author/s:
  • Alfonso Rubí, Julio
  • Ortego, Felix
  • Castañera, Pedro
  • Carbonero Zalduegui, Pilar
  • Diaz Rodriguez, Isabel
Item Type: Article
Título de Revista/Publicación: Transgenic Research
Date: February 2003
Volume: 12
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Indica and japonica rice (Oryza sativa L.) plants were transformed by particle bombardment with the Itr1 gene encoding the barley trypsin inhibitor BTI-CMe, under the control of its own promoter that confers endosperm specificity, and the maize ubiquitin promoter. From 38 independent transgenic lines of indica (breeding line IR58) and 15 of the japonica (cv Senia) selected, 22 and 11, respectively, expressed the barley inhibitor at detectable levels. The transgene was correctly translated as indicated by western blot analysis with a level of expression in R3 seeds up to 0.31% (IR58) and 0.43% (Senia) of the total extracted protein. The functional integrity of BTI-CMe was confirmed by trypsin activity assays in liquid media and by activity staining gels, performed with seed extracts. The significant reduction of the survival rate of the rice weevil (Sitophilus oryzae, Coleoptera: Curculionidae) reared on homozygous transgenic indica and japonica rice seeds expressing the BTI-CMe, compared to non-transformed controls, and the decrease in the trypsin-like activity of insect crude midgut extracts, confirmed the utility of this proteinase inhibitor gene for the control of important storage pests.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGF98-0805-C02-01UnspecifiedUnspecifiedUnspecified
Madrid Regional Government07B/0006/98UnspecifiedIsabel DíazIdentificación y mutagenación de genes inhibidores de proteasas para la obtención de plantas transgénicas resistentes a insectos

More information

Item ID: 53916
DC Identifier: http://oa.upm.es/53916/
OAI Identifier: oai:oa.upm.es:53916
DOI: 10.1023/A:1022176207180
Official URL: https://link.springer.com/article/10.1023/A:1022176207180
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 11 Feb 2019 11:32
Last Modified: 11 Feb 2019 11:34
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