A Role for the DOF Transcription Factor BPBF in the Regulation of Gibberellin-Responsive Genes in Barley Aleurone

Mena, Montaña and Cejudo, Francisco Javier and Isabel La Moneda, Inés and Carbonero Zalduegui, Pilar (2002). A Role for the DOF Transcription Factor BPBF in the Regulation of Gibberellin-Responsive Genes in Barley Aleurone. "Plant Physiology", v. 130 (n. 1); pp. 111-119. ISSN 0032-0889. https://doi.org/10.1104/pp.005561.

Description

Title: A Role for the DOF Transcription Factor BPBF in the Regulation of Gibberellin-Responsive Genes in Barley Aleurone
Author/s:
  • Mena, Montaña
  • Cejudo, Francisco Javier
  • Isabel La Moneda, Inés
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Plant Physiology
Date: September 2002
ISSN: 0032-0889
Volume: 130
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Functional analyses of a number of hydrolase gene promoters, induced by gibberellin (GA) in aleurone cells following germination, have identified a GA-responsive complex as a tripartite element containing a pyrimidine box motif 5�-CCTTTT- 3�. We describe here that BPBF, a barley (Hordeum vulgare) transcription factor of the DOF (DNA-Binding with One Finger) class, previously shown to be an activator of reserve protein encoding genes during development, also has a role in the control of hydrolase genes following seed germination. Northern-blot, reverse transcriptase-polymerase chain reaction, and in situ hybridization analyses evidenced that the transcripts of the BPBF-encoding gene (Pbf), besides being present during endosperm development, are also expressed in aleurone cells of germinated seeds where they are induced by GA, an effect counteracted by abscisic acid. Electrophoretic mobility shift assays have shown that the BPBF protein binds specifically to the pyrimidine box motif in vitro within the different sequence contexts that naturally occur in the promoters of genes encoding a cathepsin B-like protease (Al21) and a low-isoelectric point �-amylase (Amy2/32b), both induced in the aleurone layers in response to GA. In transient expression experiments, BPBF repressed transcription of the Al21 promoter in GA-treated barley aleurone layers and reverted the GAMYB-mediated activation of this protease promoter.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainPB97-0561UnspecifiedPilar Carbonero ZaldueguiRegulación genética en semillas de cereales: caracterización molecular de factores transcripcionales tipo BZIP y DOF
Government of SpainPB97-0745UnspecifiedFrancisco Javier CejudoEstudio de genes implicados en la biosíntesis y degradación de proteínas de semillas de trigo
Government of SpainBMC2000-1483UnspecifiedPilar Carbonero ZaldueguiGenes reguladores del desarrollo y la germinación de semillas de cebada y Arabidopsis

More information

Item ID: 53921
DC Identifier: http://oa.upm.es/53921/
OAI Identifier: oai:oa.upm.es:53921
DOI: 10.1104/pp.005561
Official URL: http://www.plantphysiol.org/content/130/1/111
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 11 Feb 2019 09:59
Last Modified: 11 Feb 2019 09:59
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