The GAMYB protein from barley interacts with the DOF transcription factor BPBF and activates endosperm‐specific genes during seed development

Diaz Rodriguez, Isabel and Vicente Carbajosa, Jesus and Abraham, Zamira and Martinez Muñoz, Manuel and Isabel La Moneda, Inés and Carbonero Zalduegui, Pilar (2002). The GAMYB protein from barley interacts with the DOF transcription factor BPBF and activates endosperm‐specific genes during seed development. "Plant Journal", v. 29 (n. 4); pp. 453-464. ISSN 0960-7412. https://doi.org/10.1046/j.0960-7412.2001.01230.x.

Description

Title: The GAMYB protein from barley interacts with the DOF transcription factor BPBF and activates endosperm‐specific genes during seed development
Author/s:
  • Diaz Rodriguez, Isabel
  • Vicente Carbajosa, Jesus
  • Abraham, Zamira
  • Martinez Muñoz, Manuel
  • Isabel La Moneda, Inés
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Plant Journal
Date: February 2002
Volume: 29
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

HvGAMYB, a MYB transcription factor previously shown to be expressed in barley aleurone cells in response to gibberellin during germination, also has an important role in gene regulation during endosperm development. The mRNA was detected early (10 DAF) in the seeds where it accumulates, not only in the aleurone layer, starchy endosperm, nucellar projection and vascular tissue, but also in the immature embryo as shown by in situ hybridization analysis. The HvGAMYB protein, expressed in bacteria, binds to oligonucleotides containing the 5′‐TAACAAC‐3′ or 5′‐CAACTAAC‐3′ sequences, derived from the promoter regions of the endosperm‐specific genes Hor2 and Itr1, encoding a B‐hordein and trypsin‐inhibitor BTI‐CMe, respectively. Binding is prevented when these motifs are mutated to 5′‐TgACAAg‐3′ and 5′‐CgACTgAC‐3′. Transient expression experiments in co‐bombarded developing endosperms demonstrate that HvGAMYB trans‐activates transcription from native Hor2 and Itr1 promoters through binding to the intact motifs described above. Trans‐activation of the Hor2 promoter also requires an intact prolamine box (PB). This suggests that HvGAMYB interacts in developing barley endosperms with the PB‐binding factor BPBF, an endosperm‐specific DOF transcriptional activator of the Hor2 gene. The in vivo interaction experiment between HvGAMYB and BPBF was done in the yeast two‐hybrid system, where HvGAMYB potentiates the BPBF trans‐activation capacity through interaction with its C‐terminal domain.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainPB97-0561UnspecifiedPilar Carbonero ZaldueguiRegulación genética en semillas de cereales: caracterización molecular de factores transcripcionales tipo BZIP y DOF
Government of SpainBMC2000-1483UnspecifiedPilar Carbonero ZaldueguiGenes reguladores del desarrollo y la germinación de semillas de cebada y Arabidopsis
Madrid Regional Government07G/0015/2000UnspecifiedUnspecifiedUnspecified

More information

Item ID: 53923
DC Identifier: http://oa.upm.es/53923/
OAI Identifier: oai:oa.upm.es:53923
DOI: 10.1046/j.0960-7412.2001.01230.x
Official URL: https://onlinelibrary.wiley.com/doi/10.1046/j.0960-7412.2001.01230.x
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 11 Feb 2019 09:03
Last Modified: 11 Feb 2019 09:03
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