A constitutive cystatin-encoding gene from barley (Icy) responds differentially to abiotic stimuli

Gaddour, Kamel and Vicente Carbajosa, Jesus and Lara, Pilar and Isabel La Moneda, Inés and Diaz Rodriguez, Isabel and Carbonero Zalduegui, Pilar (2001). A constitutive cystatin-encoding gene from barley (Icy) responds differentially to abiotic stimuli. "Plant Molecular Biology", v. 45 (n. 5); pp. 599-608. ISSN 0167-4412. https://doi.org/10.1023/A:1010697204686.

Description

Title: A constitutive cystatin-encoding gene from barley (Icy) responds differentially to abiotic stimuli
Author/s:
  • Gaddour, Kamel
  • Vicente Carbajosa, Jesus
  • Lara, Pilar
  • Isabel La Moneda, Inés
  • Diaz Rodriguez, Isabel
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Plant Molecular Biology
Date: March 2001
ISSN: 0167-4412
Volume: 45
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

A barley cDNA clone encoding a cysteine proteinase inhibitor was characterized. The deduced amino acid sequence of this barley cystatin (Hv-CPI) contains the motif QXVXG conserved among members of the cystatin superfamily. Thegene(Icy), located on chromosome 2, was expressed in embryos, developing endosperms, leaves and roots as assessed by northern blot analysis. Western blot analysis detected a slightly retarded band in leaves that was not present in roots or seeds. In these two organs a more precise location of Hv-CPI was done by immuno-histochemical analysis, with polyclonal antibodies raised against the recombinant CPI protein expressed in Escherichia coli. This protein efficiently inhibited papain (Ki 2.0 × 10−8 M) and ficin (Ki 2.2 × 10−8 M) and, to a lesser extent, chymopapain (Ki 1.6 × 10−7 M) and was inactive against bromelain. The Icy mRNA expression in vegetative tissues increased in response to anaerobiosis, dark and cold shock (6 ◦C).

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO96-2303UnspecifiedPilar Carbonero ZaldueguiIdentificación y mutagenización de genes de cereales para su utilización transgénica frente a plagas de insectos
Madrid Regional Government07B/0006/98UnspecifiedIsabel DíazIdentificación y mutagenización de genes inhibidores de proteasas para la obtención de plantas transgénicas resistentes a insectos

More information

Item ID: 53938
DC Identifier: http://oa.upm.es/53938/
OAI Identifier: oai:oa.upm.es:53938
DOI: 10.1023/A:1010697204686
Official URL: https://link.springer.com/content/pdf/10.1023%2FA%3A1010697204686.pdf
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 11 Feb 2019 11:41
Last Modified: 11 Feb 2019 11:41
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