Parameters influencing the regeneration capacity of calluses derived from mature indica and japonica rice seeds after microprojectile bombardment

Alfonso Rubí, Julio and Carbonero Zalduegui, Pilar and Diaz Rodriguez, Isabel (1999). Parameters influencing the regeneration capacity of calluses derived from mature indica and japonica rice seeds after microprojectile bombardment. "Euphytica", v. 107 (n. 2); pp. 115-122. ISSN 0014-2336. https://doi.org/10.1023/A:1026434228146.

Description

Title: Parameters influencing the regeneration capacity of calluses derived from mature indica and japonica rice seeds after microprojectile bombardment
Author/s:
  • Alfonso Rubí, Julio
  • Carbonero Zalduegui, Pilar
  • Diaz Rodriguez, Isabel
Item Type: Article
Título de Revista/Publicación: Euphytica
Date: June 1999
ISSN: 0014-2336
Volume: 107
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The microprojectile bombardment procedure has allowed the stable transformation of indica and japonica rice varieties, although at different frequencies of transformation depending mainly on their regeneration capacity and on the specific parameters of the transformation protocol. A study of the process of regeneration to whole plants from primary calli derived from mature indica and japonica rice seeds, via embryogenesis, has shown that somatic embryos are produced by division and differentiation of the external cell layers of callus tissues. Adjusting the bombardment conditions to optimize gene delivery to those regenerable cells, we have evaluated the influence of parameters such as the target distance, particle penetration and the effect of osmotic treatment on the regeneration capacity of bombarded cells.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO96-2303UnspecifiedPilar CarboneroIdentificación y mutagenización de genes de cereales para su utilización transgénica frente a plagas de insectos
Government of SpainBIO95-2061-EUnspecifiedPilar CarboneroArroz transgénico resistente a plagas de insectos

More information

Item ID: 53943
DC Identifier: http://oa.upm.es/53943/
OAI Identifier: oai:oa.upm.es:53943
DOI: 10.1023/A:1026434228146
Official URL: https://link.springer.com/article/10.1023/A:1026434228146
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 12 Feb 2019 09:37
Last Modified: 12 Feb 2019 09:37
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