Increased insect resistance in transgenic wheat stably expressing trypsin inhibitor CMe

Altpeter, Fredy and Diaz Rodriguez, Isabel and McAuslane, Heather and Gaddour, Kamel and Carbonero Zalduegui, Pilar and Vasil, Indra K. (1999). Increased insect resistance in transgenic wheat stably expressing trypsin inhibitor CMe. "Molecular Breeding", v. 5 (n. 1); pp. 56-63. ISSN 1380-3743. https://doi.org/10.1023/A:10096599.

Description

Title: Increased insect resistance in transgenic wheat stably expressing trypsin inhibitor CMe
Author/s:
  • Altpeter, Fredy
  • Diaz Rodriguez, Isabel
  • McAuslane, Heather
  • Gaddour, Kamel
  • Carbonero Zalduegui, Pilar
  • Vasil, Indra K.
Item Type: Article
Título de Revista/Publicación: Molecular Breeding
Date: January 1999
Volume: 5
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Proteinase inhibitors have been proposed to function as plant defence agents against herbivorous pests. We have introduced the barley trypsin inhibitor CMe (BTI-CMe) into wheat (Triticum aestivum L.) by biolistic bombardment of cultured immature embryos. Of the 30 independent transgenic wheat lines selected, 16 expressed BTI-CMe. BTI-CMe was properly transcribed and translated as indicated by northern and western blot, with a level of expression in transgenic wheat seeds up to 1.1% of total extracted protein. No expression was detected in untransformed wheat seeds. Functional integrity of BTI-CMe was confirmed by trypsin inhibitor activity assay. The significant reduction of the survival rate of the Angoumois grain moth (Sitotroga cerealella, Lepidoptera: Gelechiidae), reared on transgenic wheat seeds expressing the trypsin inhibitor BTI-CMe, compared to the untransformed control confirmed the potential of BTI-CMe for the increase of insect resistance. However, only early-instar larvae were inhibited in transgenic seeds and expression of BTI-CMe protein in transgenic leaves did not have a significant protective effect against leaf-feeding insects.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO96-2303UnspecifiedPilar Carbonero ZaldueguiIdentificación y mutagenización de genes de cereales para su utilización transgénica frente a plagas de insectos

More information

Item ID: 53944
DC Identifier: http://oa.upm.es/53944/
OAI Identifier: oai:oa.upm.es:53944
DOI: 10.1023/A:10096599
Official URL: https://link.springer.com/article/10.1023/A:1009659911798
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 12 Feb 2019 07:00
Last Modified: 12 Feb 2019 07:42
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