The Spatial Distribution of Sucrose Synthase Isozymes in Barley

Guerin, Jennifer and Carbonero Zalduegui, Pilar (1998). The Spatial Distribution of Sucrose Synthase Isozymes in Barley. "Plant Physiology", v. 114 (n. 1); pp. 55-62. ISSN 0032-0889. https://doi.org/10.1104/pp.114.1.55.

Description

Title: The Spatial Distribution of Sucrose Synthase Isozymes in Barley
Author/s:
  • Guerin, Jennifer
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Plant Physiology
Date: May 1998
ISSN: 0032-0889
Volume: 114
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The sucrose (Suc) synthase enzyme purified from barley (Hordeum vulgare L.) roots is a homotetramer that is composed of 90-kD type 1 Suc synthase (SS1) subunits. Km values for Suc and UDP were 30 mM and 5 [mu]M, respectively. This enzyme can also utilize ADP at 25% of the UDP rate. Anti-SS1 polyclonal antibodies, which recognized both SS1 and type 2 Suc synthase (SS2) (88-kD) subunits, and antibodies raised against a synthetic peptide, LANGSTDNNFV, which were specific for SS2, were used to study the spatial distribution of these subunits by immunoblot analysis and immunolocalization. Both SS1 and SS2 were abundantly expressed in endosperm, where they polymerize to form the five possible homo- and heterotetramers. Only SS1 homotetramers were detected in young leaves, where they appeared exclusively in phloem cells, and in roots, where expression was associated with cap cells and the vascular bundle. In the seed both SS1 and SS2 were present in endosperm, but only SS1 was apparent in the chalazal region, the nucellar projection, and the vascular bundle. The physiological implications for the difference in expression patterns observed are discussed with respect to the maize (Zea mays L.) model.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO93-1184UnspecifiedUnspecifiedUnspecified

More information

Item ID: 53967
DC Identifier: http://oa.upm.es/53967/
OAI Identifier: oai:oa.upm.es:53967
DOI: 10.1104/pp.114.1.55
Official URL: http://www.plantphysiol.org/content/plantphysiol/114/1/55.full.pdf
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 13 Feb 2019 07:42
Last Modified: 13 Feb 2019 07:42
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