Isolation and promoter characterization of barley gene ltr1 encoding trypsin inhibitor BTI-CMe: differential activity in wild-type and mutant lys3a endosperm

Royo, Joaquin and Diaz Rodriguez, Isabel and Rodriguez Palenzuela, Pablo and Carbonero Zalduegui, Pilar (1996). Isolation and promoter characterization of barley gene ltr1 encoding trypsin inhibitor BTI-CMe: differential activity in wild-type and mutant lys3a endosperm. "Plant Molecular Biology", v. 31 (n. 5); pp. 1051-1059. ISSN 0167-4412. https://doi.org/10.1007/BF00040723.

Description

Title: Isolation and promoter characterization of barley gene ltr1 encoding trypsin inhibitor BTI-CMe: differential activity in wild-type and mutant lys3a endosperm
Author/s:
  • Royo, Joaquin
  • Diaz Rodriguez, Isabel
  • Rodriguez Palenzuela, Pablo
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Plant Molecular Biology
Date: August 1996
ISSN: 0167-4412
Volume: 31
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The geneItr1, encoding trypsin inhibitor BTI-CMe, has been obtained from a genomic library ofHordeum vulgare L. The gene has no introns and presents in its 5-upstream region 605 bp that are homologous to the long terminal repeats (LTR) of the copia-like retro-transposon Bare-1. Functional analysis of theItr1 promoter by transient expression in protoplasts derived from different barley tissues, has shown that in this system theItr1 promoter retains its endosperm specifity and thetrans-regulation mediated by theLys3a gene. The proximal promoter extending 343 bp upstream of the translation initiation ATG codon is sufficient to confer fullGUS expression and for endosperm specifity. In protoplasts derived from thelys3a mutant, Ris 1508,GUS activity was less than 5% of that obtained with the same constructs in the protoplasts of wild-type Bomi from which it derives. Gel retardation experiments, after incubation with proteins obtained from both types of endosperm nuclei, also show differential patterns. Possible reasons for these differences are discussed.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO94-0404UnspecifiedUnspecifiedUnspecified
Government of SpainBIO91-0782UnspecifiedPilar CarboneroCaracterización molecular de una familia de inhibidores de proteasas/alfa-amilasas de cereales y utilización de los clones correspondientes para producir plantas transgénicas resistentes a insectos fitófagos

More information

Item ID: 53976
DC Identifier: http://oa.upm.es/53976/
OAI Identifier: oai:oa.upm.es:53976
DOI: 10.1007/BF00040723
Official URL: https://link.springer.com/article/10.1007/BF00040723
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 13 Feb 2019 11:38
Last Modified: 13 Feb 2019 11:38
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