The promoter of the gene ltr1 from barley confers a different tissue specificity in transgenic tobacco

Diaz Rodriguez, Isabel and Royo, Joaquin and O'Connor, Aileen and Carbonero Zalduegui, Pilar (1995). The promoter of the gene ltr1 from barley confers a different tissue specificity in transgenic tobacco. "Molecular and General Genetics MGG", v. 248 (n. 5); pp.. ISSN 0026-8925. https://doi.org/10.1007/BF02423455.

Description

Title: The promoter of the gene ltr1 from barley confers a different tissue specificity in transgenic tobacco
Author/s:
  • Diaz Rodriguez, Isabel
  • Royo, Joaquin
  • O'Connor, Aileen
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Molecular and General Genetics MGG
Date: September 1995
ISSN: 0026-8925
Volume: 248
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Tissue-specific expression of the gene coding for trypsin inhibitor BTI-CMe in barley (!tri) occurs during the first half of endosperm development. In transgenic tobacco, the Itr I promoter drives expression of the {3-glucuronidase reporter gene not only in devel­ oping endosperm but also in embryo, cotyledons and the meristematic intercotyledonary zone of germinat­ ing seedlings. A promoter fragment extending 343 bp upstream of the translation initiation ATG codon was sufficient for full transgene expression, whereas, the proximal 83 bp segment of the promoter was inactive. Possible reasons for the differences in expression patterns are discussed.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO91-0782UnspecifiedPilar CarboneroCaracterización molecular de una familia de inhibidores de proteasas/alfa-amilasas de cereales y utilización de los clones correspondientes para producir plantas transgénicas resistentes a insectos fitófagos

More information

Item ID: 53979
DC Identifier: http://oa.upm.es/53979/
OAI Identifier: oai:oa.upm.es:53979
DOI: 10.1007/BF02423455
Official URL: https://link.springer.com/article/10.1007/BF02423455
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 14 Feb 2019 08:43
Last Modified: 14 Feb 2019 08:43
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