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The promoter of the gene ltr1 from barley confers a different tissue specificity in transgenic tobacco
Citation
Diaz Rodriguez, Isabel and Royo, Joaquin and O'Connor, Aileen and Carbonero Zalduegui, Pilar (1995). The promoter of the gene ltr1 from barley confers a different tissue specificity in transgenic tobacco. "Molecular and General Genetics MGG", v. 248 (n. 5); pp.. ISSN 0026-8925. https://doi.org/10.1007/BF02423455.
Description
| Title: | The promoter of the gene ltr1 from barley confers a different tissue specificity in transgenic tobacco |
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| Author/s: |
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| Item Type: | Article |
| Título de Revista/Publicación: | Molecular and General Genetics MGG |
| Date: | September 1995 |
| ISSN: | 0026-8925 |
| Volume: | 248 |
| Subjects: | |
| Faculty: | E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM) |
| Department: | Biotecnología - Biología Vegetal |
| Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
Abstract
Tissue-specific expression of the gene coding for trypsin inhibitor BTI-CMe in barley (!tri) occurs during the first half of endosperm development. In transgenic tobacco, the Itr I promoter drives expression of the {3-glucuronidase reporter gene not only in devel oping endosperm but also in embryo, cotyledons and the meristematic intercotyledonary zone of germinat ing seedlings. A promoter fragment extending 343 bp upstream of the translation initiation ATG codon was sufficient for full transgene expression, whereas, the proximal 83 bp segment of the promoter was inactive. Possible reasons for the differences in expression patterns are discussed.
Funding Projects
| Type | Code | Acronym | Leader | Title |
|---|---|---|---|---|
| Government of Spain | BIO91-0782 | Unspecified | Pilar Carbonero | Caracterización molecular de una familia de inhibidores de proteasas/alfa-amilasas de cereales y utilización de los clones correspondientes para producir plantas transgénicas resistentes a insectos fitófagos |
More information
| Item ID: | 53979 |
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| DC Identifier: | http://oa.upm.es/53979/ |
| OAI Identifier: | oai:oa.upm.es:53979 |
| DOI: | 10.1007/BF02423455 |
| Official URL: | https://link.springer.com/article/10.1007/BF02423455 |
| Deposited by: | Biblioteca ETSI Agrónomos |
| Deposited on: | 14 Feb 2019 08:43 |
| Last Modified: | 14 Feb 2019 08:43 |
