Gene specificity is maintained in transient expression assays with protoplasts derived from different tissues of barley

Diaz Rodriguez, Isabel and Royo, Joaquin and Sanchez de la Hoz, Pilar and Carbonero Zalduegui, Pilar (1995). Gene specificity is maintained in transient expression assays with protoplasts derived from different tissues of barley. "Euphytica", v. 85 (n. 1-3); pp. 203-207. ISSN 0014-2336. https://doi.org/10.1007/BF00023949.

Description

Title: Gene specificity is maintained in transient expression assays with protoplasts derived from different tissues of barley
Author/s:
  • Diaz Rodriguez, Isabel
  • Royo, Joaquin
  • Sanchez de la Hoz, Pilar
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Euphytica
Date: February 1995
ISSN: 0014-2336
Volume: 85
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

In sorne cereal species that are still recalcitrant to stable transformation and regeneration, transient expression in isolated protoplasts is a useful tool for the study of gene expression and regulation. We have successfully applied these techniques to barley protoplasts derived from developing endosperm, aleurone, leaves and roots in order to characterize functionally cis-acting motives in two gene promoters, corresponding to trypsin inhibitor BTI-CMe and to sucrose synthase Ss1. Gene specificity is maintained in transient expression assays with protoplasts isolated from these different barley tissues and the pattem of expression parallels the mRNA levels observed for the corresponding genes in the same tissues.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO93-1184UnspecifiedUnspecifiedUnspecified
Government of SpainBIO91-0782UnspecifiedPilar CarboneroCaracterización molecular de una familia de inhibidores de proteasas/alfa-amilasas de cereales y utilización de los clones correspondientes para producir plantas transgénicas resistentes a insectos fitófagos

More information

Item ID: 53987
DC Identifier: http://oa.upm.es/53987/
OAI Identifier: oai:oa.upm.es:53987
DOI: 10.1007/BF00023949
Official URL: https://link.springer.com/article/10.1007/BF00023949
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 14 Feb 2019 08:38
Last Modified: 14 Feb 2019 08:38
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