Sharp divergence between wheat and barley at loci encoding novel members of the trypsin/ a-amylase inhibitors family

Sanchez de la Hoz, Pilar and Castagnaro, Atilio and Carbonero Zalduegui, Pilar (1994). Sharp divergence between wheat and barley at loci encoding novel members of the trypsin/ a-amylase inhibitors family. "Plant Molecular Biology", v. 26 (n. 4); pp. 1231-1236. ISSN 0167-4412. https://doi.org/10.1007/BF00040705.

Description

Title: Sharp divergence between wheat and barley at loci encoding novel members of the trypsin/ a-amylase inhibitors family
Author/s:
  • Sanchez de la Hoz, Pilar
  • Castagnaro, Atilio
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Plant Molecular Biology
Date: November 1994
ISSN: 0167-4412
Volume: 26
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (405kB) | Preview

Abstract

Amino acid sequences for three members (CMxl, CMx2, and CMx3) of a new subfamily of trypsin/Ol­ amylase inhibitors in wheat have been deduced from the nucleotide sequences of the corresponding cDNAs. A cDNA clone encoding CMx l was selected from a wheat developing endosperm library using a probe that encoded barley trypsin inhibitor BTI-CMe at low stringency. Sequences corresponding to CMx2 and CMx3 were obtained from cDNA amplified by the polymerase chain reaction. The three CMx sequences contain a premature stop codon after 363 nt, as well as a second stop codon at the same position as in BTI-CMe (nt 439-441). Southern analysis of DNAs from diploid, tetraploid, and hexap­ loid wheats, as well as from aneuploid lines, indicate that there is a single CMx locus in each of the three genomes of hexaploid wheat, respectively associated with chromosomal arms 4AS, 4BS, and 4DL. These genes are expressed early during endosperm development and not expressed at detectable levels in other tissues. Evolutionary implications are discussed.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO91-0782UnspecifiedPilar CarboneroCaracterización molecular de una familia de inhibidores de proteasas/alfa-amilisas de cereales y utilización de los clones correspondientes para producir plantas transgénicas resistentes a insectos fitófagos

More information

Item ID: 53988
DC Identifier: http://oa.upm.es/53988/
OAI Identifier: oai:oa.upm.es:53988
DOI: 10.1007/BF00040705
Official URL: https://link.springer.com/article/10.1007/BF00040705
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 14 Feb 2019 07:52
Last Modified: 14 Feb 2019 07:52
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM