Cloning of Cdna, Expression, and Chromosomal Location of Genes Encoding the 3 Types of Subunits of the Barley Tetrameric Inhibitor of Insect Alpha-Amylase

Medina Alcazar, Joaquin and Hueros, Gregorio and Carbonero Zalduegui, Pilar (1993). Cloning of Cdna, Expression, and Chromosomal Location of Genes Encoding the 3 Types of Subunits of the Barley Tetrameric Inhibitor of Insect Alpha-Amylase. "Plant Molecular Biology", v. 23 (n. 3); pp. 535-542. ISSN 0167-4412. https://doi.org/10.1007/BF00019301.

Description

Title: Cloning of Cdna, Expression, and Chromosomal Location of Genes Encoding the 3 Types of Subunits of the Barley Tetrameric Inhibitor of Insect Alpha-Amylase
Author/s:
  • Medina Alcazar, Joaquin
  • Hueros, Gregorio
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Plant Molecular Biology
Date: November 1993
ISSN: 0167-4412
Volume: 23
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Three cDNA clones from barley developing endosperm, corresponding to proteins BTAI-CMa, BTAI-CMb and BTAI-CMd, which are the three types of subunits of the tetrameric inhibitor of insect α-amylases, have been identified and sequenced. The deduced amino acid sequence of BTAI-CMb corresponds to the CM16/CM17 type of subunit in wheat (92/90% identical residues) and has one putative N-glycosylation site (NLT) and a possible kinase-C phosphorylation site (SCR). The BTAI-CMa sequence differs at four amino acid residues from a previously reported one from cv. Bomi and the sequence deduced for BTAI-CMd completes (11 N-terminal residues) and confirms previously available data. The gene for BTAI-CMa (Iat1) is located in the β arm of barley chromosome 7H (syn.1), while genes for both BTAI-CMb (Iat2) and BTAI-CMd (Iat3) are in the long arm of chromosome 4H. The three genes are expressed in endosperm and their mRNAs are not detected in the other tissues tested, except Iat1, which seems to be expressed at a low level in coleoptile and roots, where it is switched off by 50 μM methyl jasmonate.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO91-0782UnspecifiedPilar CarboneroCaracterización molecular de una familia de inhibidores de proteasas/alfa-amilasas de cereales y utilización de los clones correspondientes para producir plantas transgénicas resistentes a insectos fitófagos

More information

Item ID: 53997
DC Identifier: http://oa.upm.es/53997/
OAI Identifier: oai:oa.upm.es:53997
DOI: 10.1007/BF00019301
Official URL: https://link.springer.com/article/10.1007/BF00019301
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 14 Feb 2019 12:36
Last Modified: 14 Feb 2019 12:38
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