The promoter of barley trypsin-inhibitor BTI-CMe, discriminates between wheat and barley endosperm protoplasts in transient expression assays

Diaz Rodriguez, Isabel and Royo, Joaquin and Carbonero Zalduegui, Pilar (1993). The promoter of barley trypsin-inhibitor BTI-CMe, discriminates between wheat and barley endosperm protoplasts in transient expression assays. "Plant Cell Reports", v. 12 (n. 12); pp. 698-701. ISSN 0721-7714. https://doi.org/10.1007/BF00233422.

Description

Title: The promoter of barley trypsin-inhibitor BTI-CMe, discriminates between wheat and barley endosperm protoplasts in transient expression assays
Author/s:
  • Diaz Rodriguez, Isabel
  • Royo, Joaquin
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Plant Cell Reports
Date: October 1993
ISSN: 0721-7714
Volume: 12
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

everal promoter fragments from the barley gene coding for trypsin inhibitor, BTI-CMe, have been fused to the β-glucuronidase (GUS) reporter gene and these chimeric constructs used for transient expression in protoplasts. Transfection of developing endosperm protoplasts from barley (cv Bomi) show a maximum GUS expression of about 50% of that driven by the cauliflower mosaic virus 35S promoter, while in wheat endosperm protoplasts expression is less than 10%. No significant expression is found in transfected leaf protoplasts from barley, wheat or tobacco (<2% of the 35S control). All the information required for endosperm and barley specificity is present in the 343 bp proximal to the translation initiation site.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO91-0782UnspecifiedPilar CarboneroCaracterización molecular de una familia de inhibidores de proteasas/alfa-amilasas de cereales y utilización de los clones correspondientes para producir plantas transgénicas resistentes a insectos fitófagos
Government of SpainPB89-0190UnspecifiedPilar CarboneroCaracterización molecular de los genes de sacarosa sintética en trigo y cebada y su expresión en respuesta a factores ambientales y hormonales

More information

Item ID: 54003
DC Identifier: http://oa.upm.es/54003/
OAI Identifier: oai:oa.upm.es:54003
DOI: 10.1007/BF00233422
Official URL: https://link.springer.com/article/10.1007/BF00233422
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 14 Feb 2019 11:02
Last Modified: 14 Feb 2019 11:02
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