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Diaz Rodriguez, Isabel and Royo, Joaquin and Carbonero Zalduegui, Pilar (1993). The promoter of barley trypsin-inhibitor BTI-CMe, discriminates between wheat and barley endosperm protoplasts in transient expression assays. "Plant Cell Reports", v. 12 (n. 12); pp. 698-701. ISSN 0721-7714. https://doi.org/10.1007/BF00233422.
Title: | The promoter of barley trypsin-inhibitor BTI-CMe, discriminates between wheat and barley endosperm protoplasts in transient expression assays |
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Author/s: |
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Item Type: | Article |
Título de Revista/Publicación: | Plant Cell Reports |
Date: | October 1993 |
ISSN: | 0721-7714 |
Volume: | 12 |
Subjects: | |
Faculty: | E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM) |
Department: | Biotecnología - Biología Vegetal |
Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
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everal promoter fragments from the barley gene coding for trypsin inhibitor, BTI-CMe, have been fused to the β-glucuronidase (GUS) reporter gene and these chimeric constructs used for transient expression in protoplasts. Transfection of developing endosperm protoplasts from barley (cv Bomi) show a maximum GUS expression of about 50% of that driven by the cauliflower mosaic virus 35S promoter, while in wheat endosperm protoplasts expression is less than 10%. No significant expression is found in transfected leaf protoplasts from barley, wheat or tobacco (<2% of the 35S control). All the information required for endosperm and barley specificity is present in the 343 bp proximal to the translation initiation site.
Type | Code | Acronym | Leader | Title |
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Government of Spain | BIO91-0782 | Unspecified | Pilar Carbonero | Caracterización molecular de una familia de inhibidores de proteasas/alfa-amilasas de cereales y utilización de los clones correspondientes para producir plantas transgénicas resistentes a insectos fitófagos |
Government of Spain | PB89-0190 | Unspecified | Pilar Carbonero | Caracterización molecular de los genes de sacarosa sintética en trigo y cebada y su expresión en respuesta a factores ambientales y hormonales |
Item ID: | 54003 |
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DC Identifier: | http://oa.upm.es/54003/ |
OAI Identifier: | oai:oa.upm.es:54003 |
DOI: | 10.1007/BF00233422 |
Official URL: | https://link.springer.com/article/10.1007/BF00233422 |
Deposited by: | Biblioteca ETSI Agrónomos |
Deposited on: | 14 Feb 2019 11:02 |
Last Modified: | 14 Feb 2019 11:02 |