A Major Barley Allergen Associated with Bakers Asthma Disease is a Glycosylated Monomeric Inhibitor of Insect Alpha-Amylase - Cdna Cloning and Chromosomal Location of the Gene

Mena, Montaña and Sánchez-Monge Laguna de Rins, Rosa and Gómez, Luis and Salcedo Duran, Gabriel and Carbonero Zalduegui, Pilar (1992). A Major Barley Allergen Associated with Bakers Asthma Disease is a Glycosylated Monomeric Inhibitor of Insect Alpha-Amylase - Cdna Cloning and Chromosomal Location of the Gene. "Plant Molecular Biology", v. 20 (n. 3); pp. 451-458. ISSN 0167-4412. https://doi.org/10.1007/BF00040604.

Description

Title: A Major Barley Allergen Associated with Bakers Asthma Disease is a Glycosylated Monomeric Inhibitor of Insect Alpha-Amylase - Cdna Cloning and Chromosomal Location of the Gene
Author/s:
  • Mena, Montaña
  • Sánchez-Monge Laguna de Rins, Rosa
  • Gómez, Luis
  • Salcedo Duran, Gabriel
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Plant Molecular Biology
Date: November 1992
ISSN: 0167-4412
Volume: 20
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (10MB) | Preview

Abstract

A 14.5 kDa barley endosperm protein that is a major allergen in baker's asthma disease, as previously shown by both in vitro (IgE binding) and in vivo tests, has been identified as a glycosylated monomeric member of the multigene family of inhibitors of α-amylase/trypsin from cereals. A cDNA encoding this allergen (renamed BMAI-1) has been isolated and characterized. The deduced sequence for the mature protein, which is 132 residues long, is identical in its N-terminal end to the 20 amino acid partial sequence previously determined from the purified allergen, and fully confirms that it is a member of the multigene family of cereal inhibitors. Southern-blot analysis of wheat/barley addition lines using the insert in the BMAI-1 cDNA clone as a probe, has led to the location of the allergen gene (Iam1) in barley chromosome 2, while another related member of this protein family, the barley dimeric α-amylase inhibitor BDAI-1 gene (Iad1) has been located in chromosome 6. Iam1 is the first member of this inhibitor family in cereals to be assigned to chromosome group 2, thus extending the dispersion of genes in the family to five out of the seven homology groups of chromosomes in wheat and barley (chromosome 2, 3, 4, 6 and 7).

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBIO91-0782UnspecifiedPilar CarboneroCaracterización molecular de una familia de inhibidores de proteasas/alfa-amilasas de cereales y utilización de los clones correspondientes para producir plantas transgénicas resistentes a insectos fitófagos
Government of SpainALI89-0121UnspecifiedUnspecifiedUnspecified

More information

Item ID: 54015
DC Identifier: http://oa.upm.es/54015/
OAI Identifier: oai:oa.upm.es:54015
DOI: 10.1007/BF00040604
Official URL: https://link.springer.com/article/10.1007/BF00040604
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 15 Feb 2019 09:45
Last Modified: 15 Feb 2019 09:45
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM