Isolation of Protoplasts from Developing Barley Endosperm: a Tool for Transient Expression Studies

Diaz Rodriguez, Isabel and Carbonero Zalduegui, Pilar (1992). Isolation of Protoplasts from Developing Barley Endosperm: a Tool for Transient Expression Studies. "Plant Cell Reports", v. 10 (n. 12); pp. 595-598. ISSN 0721-7714. https://doi.org/10.1007/BF00232377.

Description

Title: Isolation of Protoplasts from Developing Barley Endosperm: a Tool for Transient Expression Studies
Author/s:
  • Diaz Rodriguez, Isabel
  • Carbonero Zalduegui, Pilar
Item Type: Article
Título de Revista/Publicación: Plant Cell Reports
Date: January 1992
ISSN: 0721-7714
Volume: 10
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

We have developed a method for the routine isolation of protoplasts from developing starchy endosperm of barley (Hordeum vulgare L.). Preplasmolysis of the intact endosperms, a low concentration of hydrolytic enzymes and gravity sedimentation before any centrifugation step, were crucial factors for a good preparation. Best yields were obtained early after pollination (8–13 days) or with mutants with low starch content. Transient expression of a reporter gene under the control of the 35S promoter, after polyethyleneglycol transfection of endosperm protoplasts, was of the same order as that found in coleoptile derived protoplasts. No significant difference in expression was found for a given tissue between cv. Bomi and its mutant Risø 1508.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainPB89-0190UnspecifiedPilar CarboneroCaracterización molecular de los genes de sacarosa sintética en trigo y cebada y su expresión en respuesta a factores ambientales y hormonales
Government of SpainBIO88-0216UnspecifiedUnspecifiedUnspecified

More information

Item ID: 54025
DC Identifier: http://oa.upm.es/54025/
OAI Identifier: oai:oa.upm.es:54025
DOI: 10.1007/BF00232377
Official URL: https://link.springer.com/article/10.1007%2FBF00232377
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 18 Feb 2019 07:59
Last Modified: 18 Feb 2019 07:59
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