Molecular-Cloning of African Swine Fever Virus-Dna

Ley, V. and Almendral, J.M. and Carbonero Zalduegui, Pilar and Beloso, A. and Viñuela Sandoval, Elisa and Talavera, A. (1984). Molecular-Cloning of African Swine Fever Virus-Dna. "Virology", v. 133 (n. 2); pp. 249-257. ISSN 0042-6822. https://doi.org/10.1016/0042-6822(84)90392-1.

Description

Title: Molecular-Cloning of African Swine Fever Virus-Dna
Author/s:
  • Ley, V.
  • Almendral, J.M.
  • Carbonero Zalduegui, Pilar
  • Beloso, A.
  • Viñuela Sandoval, Elisa
  • Talavera, A.
Item Type: Article
Título de Revista/Publicación: Virology
Date: March 1984
ISSN: 0042-6822
Volume: 133
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

African swine fever virus DNA (about 170 kbp) was cleaved with the restriction endonuclease EcoRI and most of the resulting 31 fragments were cloned in either the phage vector λWES.λB or the plasmid pBR325. Three fragments were not cloned in those vectors, the largest fragment EcoRI-A (21.2 kbp) and the two crosslinked terminal fragments, EcoRI-K′ and D′. Endonuclease SalI cut fragment EcoRI-A into three pieces which were cloned in plasmid pBR322. The two terminal EcoRI fragments were cloned after removal of the crosslinks with nuclease S1 and addition of EcoRI linkers to the fragment ends. The complete library of the cloned fragments accounted for about 98% of ASF virus genome, the missing sequences being those removed by the nuclease S1 in the process of cloning the terminal fragments.

More information

Item ID: 54027
DC Identifier: http://oa.upm.es/54027/
OAI Identifier: oai:oa.upm.es:54027
DOI: 10.1016/0042-6822(84)90392-1
Official URL: https://www.sciencedirect.com/science/article/abs/pii/0042682284903921
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 20 Feb 2019 12:32
Last Modified: 20 Feb 2019 12:32
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