In vitro caecal fermentation of carbohydrate-rich feedstuffs in rabbits as affected by substrate pre-digestion and donors' diet

Ocasio Vega, C. and Abad Guaman, Rodrigo and Delgado, R. and Carabaño Luengo, Rosa Maria and Carro Travieso, Maria Dolores and Garcia Alonso, Javier (2018). In vitro caecal fermentation of carbohydrate-rich feedstuffs in rabbits as affected by substrate pre-digestion and donors' diet. "World Rabbit Science", v. 26 (n. 1); pp. 15-25. ISSN 1257-5011. https://doi.org/10.4995/wrs.2018.7854.

Description

Title: In vitro caecal fermentation of carbohydrate-rich feedstuffs in rabbits as affected by substrate pre-digestion and donors' diet
Author/s:
  • Ocasio Vega, C.
  • Abad Guaman, Rodrigo
  • Delgado, R.
  • Carabaño Luengo, Rosa Maria
  • Carro Travieso, Maria Dolores
  • Garcia Alonso, Javier
Item Type: Article
Título de Revista/Publicación: World Rabbit Science
Date: 2018
ISSN: 1257-5011
Volume: 26
Subjects:
Freetext Keywords: gas production; processing procedure; donors’ diet; rabbits; caecal fermentation; volatile fatty acids
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The influence of substrate pre-digestion and donors’ diet on in vitro caecal fermentation of different substrates in rabbits was investigated. Eight crossbreed rabbits were fed 2 experimental diets containing either low (LSF; 84.0 g/kg dry matter [DM]) or high soluble fibre (HSF; 130 g/kg DM) levels. In vitro incubations were conducted using batch cultures with soft faeces as inoculum and four fibrous or fibre-derived, lowstarch and low-protein substrates: D-cellobiose (CEL), sugar beet pectin (PEC), sugar beet pulp (SBP) and wheat straw (WS). Substrates in half of the cultures were subjected to a 2-step pepsin/pancreatin in vitro digestion without filtration, and the whole residue (soluble, insoluble and added enzymes) was incubated at 39°C. Gas production was measured until 144 h, and volatile fatty acid (VFA) production at 24 h incubation was determined. Cultures without substrate (blanks) were included to correct gas production values for gas released from endogenous substrates and added enzymes. Pre-digestion had no influence on in vitro gas production kinetic of WS, and only reduced the time before gas production begins (lag time; by 31%; P=0.042) for SBP, but for both substrates the pre-digestion decreased the molar proportion of acetate (by 9%; P≤0.003) and increased those of propionate and butyrate (P≤0.014). For CEL, the pre-digestion increased the gas and total VFA production (by 30 and 114%), shortened the lag time (by 32%), and only when it was combined with LSF inoculum 38 percentage units of acetate were replaced by butyrate (P≤0.039). Treatments had a minor influence on in vitro fermentation traits of SBP pectin. The results showed that the pre-digestion process influenced the in vitro caecal fermentation in rabbits, but the effects were influenced by donors’ diet and the incubated substrate. Pre-digestion of substrate is recommended before conducting in vitro caecal fermentations. The level of soluble fibre in the donors’ diet also influenced the in vitro caecal fermentation, but its effect depended on the type of substrate.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2011-23885UnspecifiedUnspecifiedNutrientes immunoestimuladores en gazapos
Government of SpainAGL2011-22628UnspecifiedUnspecifiedEstudio de la microbiota en sistemas in vitro de simulación de la fermentación ruminal (cultivos no renovados, fermentadores rusitec y fermentadores de flujo continuo)
Madrid Regional GovernmentS2013/ABI-2913MEDGANUnspecifiedTecnologías de manejo, nutrición y genética para optimizar la producción de alimentos de origen animal característicos de la dieta mediterránea

More information

Item ID: 54588
DC Identifier: http://oa.upm.es/54588/
OAI Identifier: oai:oa.upm.es:54588
DOI: 10.4995/wrs.2018.7854
Official URL: https://polipapers.upv.es/index.php/wrs/article/view/7854/9740
Deposited by: Memoria Investigacion
Deposited on: 10 Apr 2019 11:23
Last Modified: 10 Apr 2019 11:23
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