Volume electron microscopy of the distribution of synapses in the neuropil of the juvenile rat somatosensory cortex

Santuy Muñoz, Andrea and Rodríguez, J. R. and DeFelipe, Javier and Merchán Pérez, Ángel (2018). Volume electron microscopy of the distribution of synapses in the neuropil of the juvenile rat somatosensory cortex. "Brain Structure & Function", v. 223 (n. 1); pp. 77-90. ISSN 1863-2653. https://doi.org/10.1007/s00429-017-1470-7.

Description

Title: Volume electron microscopy of the distribution of synapses in the neuropil of the juvenile rat somatosensory cortex
Author/s:
  • Santuy Muñoz, Andrea
  • Rodríguez, J. R.
  • DeFelipe, Javier
  • Merchán Pérez, Ángel
Item Type: Article
Título de Revista/Publicación: Brain Structure & Function
Date: January 2018
ISSN: 1863-2653
Volume: 223
Subjects:
Freetext Keywords: Excitatory synapses; Dendritic shafts; Somatosensory cortex; Dendritic spines; Inhibitory synapses; Serial section three-dimensional reconstruction; Dual-beam electron microscopy; FIB/SEM
Faculty: E.T.S. de Ingenieros Informáticos (UPM)
Department: Arquitectura y Tecnología de Sistemas Informáticos
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Knowing the proportions of asymmetric (excitatory) and symmetric (inhibitory) synapses in the neuropil is critical for understanding the design of cortical circuits. We used focused ion beam milling and scanning electron microscopy (FIB/SEM) to obtain stacks of serial sections from the six layers of the juvenile rat (postnatal day 14) somatosensory cortex (hindlimb representation). We segmented in three-dimensions 6184 synaptic junctions and determined whether they were established on dendritic spines or dendritic shafts. Of all these synapses, 87?94% were asymmetric and 6?13% were symmetric. Asymmetric synapses were preferentially located on dendritic spines in all layers (80?91%) while symmetric synapses were mainly located on dendritic shafts (62?86%). Furthermore, we found that less than 6% of the dendritic spines establish more than one synapse. The vast majority of axospinous synapses were established on the spine head. Synapses on the spine neck were scarce, although they were more common when the dendritic spine established multiple synapses. This study provides a new large quantitative dataset that may contribute not only to the knowledge of the ultrastructure of the cortex, but also towards defining the connectivity patterns through all cortical layers.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainSAF2015-66603-PUnspecifiedUnspecifiedEstudio de la microorganización de la corteza cerebral en pacientes de Alzheimer y del hamster como modelo para estudiar la fosforilación de tau
FP7604102HBPUnspecifiedThe Human Brain Project
Horizon 2020720270HBP SGA1UnspecifiedHuman Brain Project Specific Grant Agreement 1

More information

Item ID: 54648
DC Identifier: http://oa.upm.es/54648/
OAI Identifier: oai:oa.upm.es:54648
DOI: 10.1007/s00429-017-1470-7
Official URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5772167/pdf/429_2017_Article_1470.pdf
Deposited by: Memoria Investigacion
Deposited on: 24 Apr 2019 09:05
Last Modified: 24 Apr 2019 09:05
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