a-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytes

Arias Alvárez, M. and García García, R. M. and López Tello, J. and García Rebollar, Pilar and Gutiérrez Adán, A. and Lorenzo, P.L. (2018). a-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytes. "Reproduction, Fertility and Development", v. 30 (n. 12); pp. 1728-1738. ISSN 1031-3613. https://doi.org/10.1071/RD17525.

Description

Title: a-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytes
Author/s:
  • Arias Alvárez, M.
  • García García, R. M.
  • López Tello, J.
  • García Rebollar, Pilar
  • Gutiérrez Adán, A.
  • Lorenzo, P.L.
Item Type: Article
Título de Revista/Publicación: Reproduction, Fertility and Development
Date: November 2018
ISSN: 1031-3613
Volume: 30
Subjects:
Freetext Keywords: blastocyst rate; cell death; laboratory animal; oocyte maturation; redox status; vitamin E
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Producción Agraria
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The developmental competence of in vitro maturation (IVM) oocytes can be enhanced by antioxidant agents. The present study investigated, for the first time in the rabbit model, the effect of adding a-tocopherol (0, 100, 200 and 400 mM) during IVM on putative transcripts involved in antioxidant defence (superoxide dismutase 2, mitochondrial (SOD2), glutathione peroxidase 1 (GPX1), catalase (CAT)), cell cycle regulation and apoptosis cascade (apoptosistumour protein 53 (TP53), caspase 3, apoptosis-related cysteine protease (CASP3)), cell cycle progression (cellular cycle V-Akt murine thymoma viral oncogene homologue 1 (AKT1)), cumulus expansion (gap junction protein, alpha 1, 43 kDa (GJA1) and prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclo-oxygenase) (PTGS2)) and metabolism (glucose-6-phosphate dehydrogenase (G6PD)). Meiotic progression, mitochondrial reallocation, cumulus cell apoptosis and the developmental competence of oocytes after IVF were also assessed. Expression of SOD2, CAT, TP53, CASP3 and GJA1 was downregulated in cumulus–oocyte complexes (COCs) after IVM with 100 mM a-tocopherol compared with the group without the antioxidant. The apoptotic rate and the percentage of a non-migrated mitochondrial pattern were lower in COCs cultured with 100 mM a-tocopherol, consistent with better-quality oocytes. In fact, early embryo development was improved when 100 mM a-tocopherol was included in the IVM medium, but remained low compared with in vivomatured oocytes. In conclusion, the addition of 100 mM a-tocopherol to the maturation medium is a suitable approach to manage oxidative stress and apoptosis, as well as for increasing the in vitro developmental competence of rabbit oocytes.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2015-66145UnspecifiedUnspecifiedFunción del procesamiento alternativo del pre-ARNm en la regulación del desarrollo embrionario, determinación del sexo, fertilidad y adaptación
Government of SpainAGL2015-65572-C2-2-RUnspecifiedUnspecifiedEfecto de la restricción alimentaria materna en la respuesta ovárica y embrionaria y del papel del factor de crecimiento nervioso (NGF) tras su caracterización en el conejo
Madrid Regional GovernmentS2013/ABI-2913MEDGANUnspecifiedTecnologías de manejo, nutrición y genética para optimizar la producción de alimentos de origen animal característicos de la dieta mediterránea

More information

Item ID: 54672
DC Identifier: http://oa.upm.es/54672/
OAI Identifier: oai:oa.upm.es:54672
DOI: 10.1071/RD17525
Official URL: http://www.publish.csiro.au/rd/RD17525
Deposited by: Memoria Investigacion
Deposited on: 06 May 2019 12:39
Last Modified: 10 May 2019 11:29
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