New insights on a NGF-mediated pathway to induce ovulation in rabbits (Oryctolagus cuniculus)

Maranesi, Margherita and Petrucci, Linda and Leonardi, Leonardo and Piro, Federica and García Rebollar, Pilar and Millán, Pilar and Cocci, Paolo and Vullo, Cecilia and Parillo, Francesco and Moura, Arlindo and González Mariscal, Gabriela and Boiti, Cristiano and Zerani, Massimo (2018). New insights on a NGF-mediated pathway to induce ovulation in rabbits (Oryctolagus cuniculus). "Biology of Reproduction", v. 98 (n. 5); pp. 634-643. ISSN 0006-3363. https://doi.org/10.1093/biolre/ioy041.

Description

Title: New insights on a NGF-mediated pathway to induce ovulation in rabbits (Oryctolagus cuniculus)
Author/s:
  • Maranesi, Margherita
  • Petrucci, Linda
  • Leonardi, Leonardo
  • Piro, Federica
  • García Rebollar, Pilar
  • Millán, Pilar
  • Cocci, Paolo
  • Vullo, Cecilia
  • Parillo, Francesco
  • Moura, Arlindo
  • González Mariscal, Gabriela
  • Boiti, Cristiano
  • Zerani, Massimo
Item Type: Article
Título de Revista/Publicación: Biology of Reproduction
Date: May 2018
ISSN: 0006-3363
Volume: 98
Subjects:
Freetext Keywords: growth factors; ovary; ovulation; pituitary; seminal plasma; uterus
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Producción Agraria
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

To investigate the ovulatory mechanisms triggered by raw semen (RS) in rabbits, we examined the expression of nerve growth factor (NGF)—a supposed ovulation-inducing factor (OIF)—and cognate receptors in anterior pituitary, ovary, and cervix as well as plasma NGF and luteinizing hormone (LH) concentrations. Six does/group were sham-inseminated with sterile saline (PBS), naturally mated (NM), inseminated with RS alone or after lumbar anesthesia (ARS), or treatment with COX inhibitors (CIRS). Immunohistochemistry revealed positive signals for NGF and receptors in all tissues. RT-PCR confirmed the presence of the target transcripts in the same tissues, except NTRK1 in the cervix. Circulating NGF concentrations rose 3- to 6-fold (P < 0.01) 15 min after semen deposition into the genital tract of NM, RS, and ARS rabbits and remained sustained thereafter. Circulating NGF was 4-fold lower (P < 0.01) in CIRS than in RS does indicating that NGF is mainly synthesized by the uterus. A concomitant rise of LH and NGF concentrations was found in 83.3%, 50.0%, and 16.7% of NM, RS, and CIRS does, respectively, but not in ARS (despite high NGF circulating levels). Seminal plasma NGF concentration was 151.9 ± 9.25 μg/mL. The ovulatory responses were 0%, 83.3%, 66.7%, 16.7%, and 0% in PBS, NM, RS, ARS, and CIRS groups, respectively. Present data confirm that, although RS may induce ovulation via endocrine mechanisms through binding to NGF receptors in the ovary, a novel OIF-mediated neural mechanism facilitates ovulation in rabbits.

More information

Item ID: 54686
DC Identifier: http://oa.upm.es/54686/
OAI Identifier: oai:oa.upm.es:54686
DOI: 10.1093/biolre/ioy041
Official URL: https://academic.oup.com/biolreprod/article/98/5/634/4847873
Deposited by: Memoria Investigacion
Deposited on: 15 Jul 2019 10:19
Last Modified: 15 Jul 2019 10:19
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