Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis

Bustillo Avendaño, Estefano and Ibañez, Sergio and Periañez Rodriguez, Juan and Pozo Benito, Juan Carlos del and Moreno Risueño, Miguel Angel and Ibañez, Sergio and Sanz, Oscar and Sousa Barros, Jessica Aline and Micol, José Luis and Peréz Peréz, Jose Manuel (2018). Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis. "Plant Physiology", v. 176 (n. 2); pp. 1709-1727. ISSN 0032-0889. https://doi.org/10.1104/pp.17.00980.

Description

Title: Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis
Author/s:
  • Bustillo Avendaño, Estefano
  • Ibañez, Sergio
  • Periañez Rodriguez, Juan
  • Pozo Benito, Juan Carlos del
  • Moreno Risueño, Miguel Angel
  • Ibañez, Sergio
  • Sanz, Oscar
  • Sousa Barros, Jessica Aline
  • Micol, José Luis
  • Peréz Peréz, Jose Manuel
Item Type: Article
Título de Revista/Publicación: Plant Physiology
Date: February 2018
ISSN: 0032-0889
Volume: 176
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Body regeneration through formation of new organs is a major question in developmental biology. We investigated de novo root formation using whole leaves of Arabidopsis (Arabidopsis thaliana). Our results show that local cytokinin biosynthesis and auxin biosynthesis in the leaf blade followed by auxin long-distance transport to the petiole leads to proliferation of J0121-marked xylem-associated tissues and others through signaling of INDOLE-3-ACETIC ACID INDUCIBLE28 (IAA28), CRANE (IAA18), WOODEN LEG, and ARABIDOPSIS RESPONSE REGULATORS1 (ARR1), ARR10, and ARR12. Vasculature proliferation also involves the cell cycle regulator KIP-RELATED PROTEIN2 and ABERRANT LATERAL ROOT FORMATION4, resulting in a mass of cells with rooting competence that resembles callus formation. Endogenous callus formation precedes specification of postembryonic root founder cells, from which roots are initiated through the activity of SHORT-ROOT, PLETHORA1 (PLT1), and PLT2. Primordia initiation is blocked in shr plt1 plt2 mutant. Stem cell regulators SCHIZORIZA, JACKDAW, BLUEJAY, and SCARECROW also participate in root initiation and are required to pattern the new organ, as mutants show disorganized and reduced number of layers and tissue initials resulting in reduced rooting. Our work provides an organ regeneration model through de novo root formation, stating key stages and the primary pathways involved.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBFU2016-80315-PUnspecifiedUnspecifiedNuevos mecanismos que especifican y regeneran órganos postembrionarios en la raíz de arabidopsis
Government of SpainBFU2013-41160-PUnspecifiedUnspecifiedIdentificación y caracterización de los determinantes moleculares de la pluripotencia adulta en plantas usando la raíz de arabidopsis
FP7322082NEW ROOT STEM CELLSUnspecifiedA molecular, genomics and genetic analysis of new stem cell formation during root morphogenesis
Government of SpainAGL2012-33610UnspecifiedUnspecifiedRequisitos hormonales y genéticos de la formación de raíces adventicias: una aproximación multidisciplinar
Government of SpainBIO2015-64255-RUnspecifiedUnspecifiedGenómica comparada de la formación de raíces adventicias en tomate y clavel
Government of SpainBIO2014-52091-RUnspecifiedUnspecifiedIdentificación de nuevos genes y productos bio-activos para la optimización de los recursos naturales dentro una agricultura sostenible

More information

Item ID: 54925
DC Identifier: http://oa.upm.es/54925/
OAI Identifier: oai:oa.upm.es:54925
DOI: 10.1104/pp.17.00980
Official URL: http://www.plantphysiol.org/content/176/2/1709
Deposited by: Memoria Investigacion
Deposited on: 19 Jul 2019 11:14
Last Modified: 19 Jul 2019 11:14
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