3D Printed Structures Filled with Carbon Fibers and Functionalized with Mesenchymal Stem Cell Conditioned Media as In Vitro Cell Niches for Promoting Chondrogenesis

García-Ruíz, Josefa Predestinación and Díaz Lantada, Andrés (2018). 3D Printed Structures Filled with Carbon Fibers and Functionalized with Mesenchymal Stem Cell Conditioned Media as In Vitro Cell Niches for Promoting Chondrogenesis. "Materials", v. 11 (n. 1); pp. 1-14. ISSN 1996-1944. https://doi.org/10.3390/ma11010023.

Description

Title: 3D Printed Structures Filled with Carbon Fibers and Functionalized with Mesenchymal Stem Cell Conditioned Media as In Vitro Cell Niches for Promoting Chondrogenesis
Author/s:
  • García-Ruíz, Josefa Predestinación
  • Díaz Lantada, Andrés
Item Type: Article
Título de Revista/Publicación: Materials
Date: January 2018
ISSN: 1996-1944
Volume: 11
Subjects:
Freetext Keywords: tissue engineering; scaffolds for tissue repair; stem cell niches; cell culture niches; stem cell conditioned medium; carbon fiber; rapid prototyping; additive manufacture; computer-aided design & engineering
Faculty: E.T.S.I. Industriales (UPM)
Department: Ingeniería Mecánica
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

In this study, we present a novel approach towards the straightforward, rapid, and low-cost development of biomimetic composite scaffolds for tissue engineering strategies. The system is based on the additive manufacture of a computer-designed lattice structure or framework, into which carbon fibers are subsequently knitted or incorporated. The 3D-printed lattice structure acts as support and the knitted carbon fibers perform as driving elements for promoting cell colonization of the three-dimensional construct. A human mesenchymal stem cell (h-MSC) conditioned medium (CM) is also used for improving the scaffold’s response and promoting cell adhesion, proliferation, and viability. Cell culture results—in which scaffolds become buried in collagen type II—provide relevant information regarding the viability of the composite scaffolds used and the prospective applications of the proposed approach. In fact, the advanced composite scaffold developed, together with the conditioned medium functionalization, constitutes a biomimetic stem cell niche with clear potential, not just for tendon and ligament repair, but also for cartilage and endochondral bone formation and regeneration strategies.

More information

Item ID: 54949
DC Identifier: http://oa.upm.es/54949/
OAI Identifier: oai:oa.upm.es:54949
DOI: 10.3390/ma11010023
Official URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5793521/
Deposited by: Memoria Investigacion
Deposited on: 14 May 2019 15:25
Last Modified: 14 May 2019 15:25
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