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Mirea, Teona and Olivares Roza, Jimena and Yañez Mo, María and Valés Gómez, Mar and Iborra Grau, Enrique (2018). First steps towards simultaneous isolation and detection of exosomes with carbon nanotube-based SMRs. In: "IEEE International Ultrasonics Symposium (IUS 2018)", 22/10/2018 - 25/10/2018, Kobe, Japan. pp. 1-4. https://doi.org/10.1109/ULTSYM.2018.8579718.
Title: | First steps towards simultaneous isolation and detection of exosomes with carbon nanotube-based SMRs |
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Author/s: |
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Item Type: | Presentation at Congress or Conference (Article) |
Event Title: | IEEE International Ultrasonics Symposium (IUS 2018) |
Event Dates: | 22/10/2018 - 25/10/2018 |
Event Location: | Kobe, Japan |
Title of Book: | IEEE International Ultrasonics Symposium (IUS 2018) |
Título de Revista/Publicación: | IEEE International Ultrasonics Symposium (IUS 2018) |
Date: | 2018 |
ISSN: | 1051-0117 |
Subjects: | |
Freetext Keywords: | Carbon nanotubes; solidly mounted resonator; biosensor; antibody |
Faculty: | E.T.S.I. Telecomunicación (UPM) |
Department: | Ingeniería Electrónica |
Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
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Exosomes are small (20-150nm) intercellular vesicles carrying valuable information regarding disease presence or evolution. Their isolation from body fluids is one of the most challenging targets. Although many techniques already exist, these are still inefficient, expensive and time-consuming. One of these new methods is nanofiltration. Within this technique, carbon nanotube (CNT) forests have been proposed as good candidates owing to their easily controllable tube separation and growth. Previous works have proven the isolation of different particles; however, optical read-out methods are still needed to confirm the success of the process. Here we propose and start studying the integration of CNT forests in solidly mounted resonators to simultaneously isolate exosomes and gravimetrically estimate the trapped amount. As a first step we prove the ability of our devices to trap and detect CD63 antibodies, due to their high affinity to exosomes. We demonstrate a frequency shift of around 280±8 kHz with an antibody concentration of 8 nM, four times lower than previously reported concentrations used for the detection of antibodies with thin film acoustic resonators, for similar frequency shifts.
Type | Code | Acronym | Leader | Title |
---|---|---|---|---|
Government of Spain | TEC2017-84817-C2-1-R | Unspecified | Unspecified | Sensores gravimétricos de gases basados en resonadores electroacústicos de película delgada de ALN para aplicaciones en temperaturas extremas |
Government of Spain | SAF2015-69169-R | Unspecified | Unspecified | Las células natural killer y las nanovesículas extracelulares tumorales en la respuesta inmunitaria frente al cáncer humano |
Government of Spain | BIO2017-86500-R | Unspecified | Unspecified | Estudio del potencial biotecnológico de herramientas frente a tetraspaninas en cáncer, secreción de exosomas y agentes vacunales |
Madrid Regional Government | B2017/BMD-3733 | IMMUNOTHERCAN-CM | Unspecified | Unspecified |
Item ID: | 55072 |
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DC Identifier: | http://oa.upm.es/55072/ |
OAI Identifier: | oai:oa.upm.es:55072 |
DOI: | 10.1109/ULTSYM.2018.8579718 |
Official URL: | https://ieeexplore.ieee.org/document/8579718 |
Deposited by: | Memoria Investigacion |
Deposited on: | 27 May 2019 16:08 |
Last Modified: | 27 May 2019 16:08 |