Barley cystain variants against phytopathogenic fungi pests and their impact on natural enemies

Martinez Muñoz, Manuel and Alvarez Alfageme, Fernando and Castañera, Pedro and Carbonero Zalduegui, Pilar and Ortego, Felix and Diaz Rodriguez, Isabel (2005). Barley cystain variants against phytopathogenic fungi pests and their impact on natural enemies. In: "Ecological Impact of Genetically Modified Organisms", 01/06/2005-03/06/2005, Lérida, España. ISBN 92-9067-188-0. p. 6.

Description

Title: Barley cystain variants against phytopathogenic fungi pests and their impact on natural enemies
Author/s:
  • Martinez Muñoz, Manuel
  • Alvarez Alfageme, Fernando
  • Castañera, Pedro
  • Carbonero Zalduegui, Pilar
  • Ortego, Felix
  • Diaz Rodriguez, Isabel
Item Type: Presentation at Congress or Conference (Article)
Event Title: Ecological Impact of Genetically Modified Organisms
Event Dates: 01/06/2005-03/06/2005
Event Location: Lérida, España
Title of Book: Ecological Impact of Genetically Modified Organisms: Proceedings of the meeting
Date: June 2005
ISBN: 92-9067-188-0
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The goal of this study has been to know the effects of the barley cystatin Hv-CPI and seven derived variants generated by direct-mutagenesis, on the growth and digestive physiology of the Colorado potato beetle (CPB), Leptinotarsa decemlineata and to assess the potential impact of these proteins on the spiner soldier bug (SSB), Podisus maculiventris, a generalist hemipteran predator. Among the different cystatins tested, the variant C68 G, in which the only cysteine residue was changed to glycine, showed the highest inhibitory activity when tested in vitro against commercial cysteine proteases and CPB digestive enzymes. Feeding trials conducted with CPB larvae reared on transgenic potato plants expressing this variant, resulted in significant lower weight gains compared to those fed on non-transformed plants. No effects on survival, development, and weight, were observed when SSB nymphs fed on prey CPB reared with transgenic potato plants. To investigate the physiological background, biochemical analysis were carried out on guts of insects dissected at the end of the feeding assays. The effects of the barley inhibitor Hv-CPI and its variants on the growth of the the necrotrophic fungus Fusarium oxysporum have been also analysed. The cystatin Hv-CPI inhibited fungal spore germination by 25%, the five point mutations inhibited spore germination by 18 to 40%, while the two truncated forms had no antifungal effect.

Funding Projects

TypeCodeAcronymLeaderTitle
Madrid Regional Government07M/0050/2002UnspecifiedIsabel DíazEvaluación y especificidad de inhibidores de proteasas de origen vegetal frente a hongos fitopatógenos, insectos plaga y enemigos naturales
Madrid Regional Government07M/0051/2002UnspecifiedUnspecifiedUnspecified

More information

Item ID: 55357
DC Identifier: http://oa.upm.es/55357/
OAI Identifier: oai:oa.upm.es:55357
Official URL: https://www.iobc-wprs.org/
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 05 Jun 2019 12:22
Last Modified: 05 Jun 2019 12:22
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