The endophytic fungus Rhodotorula-P5 induces a priming effect in Ulmus minor promoting the protection against Ophiostoma novo-ulmi

Sobrino Plata, Juan and Ormeño Moncalvillo, Sara Maria and Fernández, Iván and Pieterse, Corné and Collada Collada, Maria Carmen and Gil Sanchez, Luis Alfonso and Martín García, Juan Antonio (2018). The endophytic fungus Rhodotorula-P5 induces a priming effect in Ulmus minor promoting the protection against Ophiostoma novo-ulmi. In: "PROGRAM & BOOK OF ABSTRACTS. LIFE+ ELMIAS Ash and Elm, and IUFRO WP 7.02.01 Rootand Stem Rots Conference (LIFE-IUFRO)", 26 Agosto-1 Septiembre 2018, Uppsala. p. 69.

Description

Title: The endophytic fungus Rhodotorula-P5 induces a priming effect in Ulmus minor promoting the protection against Ophiostoma novo-ulmi
Author/s:
  • Sobrino Plata, Juan
  • Ormeño Moncalvillo, Sara Maria
  • Fernández, Iván
  • Pieterse, Corné
  • Collada Collada, Maria Carmen
  • Gil Sanchez, Luis Alfonso
  • Martín García, Juan Antonio
Item Type: Presentation at Congress or Conference (Other)
Event Title: PROGRAM & BOOK OF ABSTRACTS. LIFE+ ELMIAS Ash and Elm, and IUFRO WP 7.02.01 Rootand Stem Rots Conference (LIFE-IUFRO)
Event Dates: 26 Agosto-1 Septiembre 2018
Event Location: Uppsala
Title of Book: PROGRAM & BOOK OF ABSTRACTS. LIFE+ ELMIAS Ash and Elm, and IUFRO WP 7.02.01 Root and Stem Rots Conference (LIFE-IUFRO)
Date: 2018
Subjects:
Freetext Keywords: Hongos endofíticos, Ulmus minor.
Faculty: E.T.S.I. Montes, Forestal y del Medio Natural (UPM)
Department: Sistemas y Recursos Naturales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (243kB) | Preview
[img] Other
Download (8MB)

Abstract

The antagonism of certain endophytes against the pathogenic fungus Ophiostoma novo-ulmi, causal agent of Dutch elm disease (DED), suggests they could be involved in tree tolerance mechanisms. To test this function, in a first trial plantlets of tolerant and susceptible elms to DED were inoculated with O. novo-ulmi and Rhodotorula sp. strain P5 (putative antagonistic endophyte) separately. In order to compare the plant responses to both, the endophyte and the pathogen, the presence of both fungi and the expression of 13 genes were quantified in the shoots of these plants. A typical defense response mediated by salicylic acid was observed in all genotypes with both fungi, regardless of their degree of tolerance. However, this response was greater with the pathogen inoculation. In a second trial, a third treatment was incorporated: the pre-inoculation with P5 one week before O. novoulmi inoculation. On this occasion, besides quantifying the presence of both fungi and analyzing the expression of the same genes, total phenols and flavonoids contents, and oxidative stress were measured. On the one hand, all genotypes showed an overexpression of different genes when they were inoculated with the pathogen, among them- were those that code for pathogenesis-related (PR) proteins. On the other hand, the overexpression was non-existent or reduced with the dual inoculation treatment. The positive influence of the pre-inoculation with P5 was also observed in the rest of analyses, e.g. the oxidative stress and concentration of total phenols and flavonoids increased in response to the pathogen inoculation, while no difference compared to control plants was observed in P5-Ophiostoma inoculated plants. As a conclusion, the defense responses induced by RhodotorulaP5 could promote priming in U. minor plants, contributing to the enhancement of plant tolerance against DED.

More information

Item ID: 55510
DC Identifier: http://oa.upm.es/55510/
OAI Identifier: oai:oa.upm.es:55510
Deposited by: Memoria Investigacion
Deposited on: 24 Jun 2019 11:08
Last Modified: 24 Jun 2019 11:08
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM