DNA methylation changes after cryopreservation: MSAP analysis by multivariant statistical methods

Martín Fernández, María Carmen and Álvarez Mari, Ana and Rodríguez, Héctor and González Benito, Elena and Ibáñez Ruiz, Miguel Ángel (2018). DNA methylation changes after cryopreservation: MSAP analysis by multivariant statistical methods. In: "Annual Meeting of the Society for Low Temperature Biology", 06/09/2018-07/09/2018, Praga, República Checa. p. 1.

Description

Title: DNA methylation changes after cryopreservation: MSAP analysis by multivariant statistical methods
Author/s:
  • Martín Fernández, María Carmen
  • Álvarez Mari, Ana
  • Rodríguez, Héctor
  • González Benito, Elena
  • Ibáñez Ruiz, Miguel Ángel
Item Type: Presentation at Congress or Conference (Other)
Event Title: Annual Meeting of the Society for Low Temperature Biology
Event Dates: 06/09/2018-07/09/2018
Event Location: Praga, República Checa
Title of Book: Proceedings of the Annual Meeting of the Society for Low Temperature Biology
Date: September 2018
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Cryopreservation allows long-term conservation, and the genetic stability of the preserved material is one of its most important features compared to other conservation techniques. However, this stability has been questioned and in some cases epi-genetic instability after cryopreservation has been detected. Epigenetic information includes heritable signals that modulate gene expression but are not encoded in the primary nucleotide sequence. DNA methylation is the most extensively studied epigenetic modification and it has been connected to many crucial biological processes. The central issue of this work was to detect epigenetic changes in the cytosine methylation pattern throughout the whole cryopreservation process in an encapsulation-dehydration protocol in Mentha xpiperita apices. Although many techniques have been described for analysing DNA methylation, MSAP (methylation sensitive amplified polymorphism) is widely used. The global DNA methylation status has been stud ied after each step of the cryopreservation protocol. The effect of the addition during the procedure of vitamin E or ascorbic acid was also evaluated. AII the methylation variations detected were statistically analysed via multinomial logistic regression in order to test their significance. This study intends to introduce a new statistical approach to address methylation data in this contexto The results obtained showed and statistically proved that significant differences in the methylation pattern existed among the steps of the protocol.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2015-64899-C2-1UnspecifiedUnspecifiedUnspecified
Government of SpainAGL2010-21989-C02-01UnspecifiedUniversidad Politécnica de MadridEstudios sobre los procesos de crioconservación en menta

More information

Item ID: 55518
DC Identifier: http://oa.upm.es/55518/
OAI Identifier: oai:oa.upm.es:55518
Official URL: https://www.sltb.info/meetings/2018/9/20/sltb-2018-at-crop-research-institute-prague
Deposited by: Memoria Investigacion
Deposited on: 18 Jul 2019 11:30
Last Modified: 18 Jul 2019 11:30
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