The presence of Rhodotorula sp. in Ulmus minor plantlets moderates the response to Ophiostoma novo-ulmi in roots of tolerant clones

Sobrino Plata, Juan and Collada Collada, Maria Carmen and Pieterse, C. M. J. and Gil Sanchez, Luis Alfonso and Martin Garcia, Juan Antonio (2018). The presence of Rhodotorula sp. in Ulmus minor plantlets moderates the response to Ophiostoma novo-ulmi in roots of tolerant clones. In: "LIFE+ ELMIAS Ash and Elm, and IUFRO WP 7.02.01 Root and Stem Rots Conference (LIFE-IUFRO)", 26 Agosto - 1 Septiembre, Uppsala. p. 101.

Description

Title: The presence of Rhodotorula sp. in Ulmus minor plantlets moderates the response to Ophiostoma novo-ulmi in roots of tolerant clones
Author/s:
  • Sobrino Plata, Juan
  • Collada Collada, Maria Carmen
  • Pieterse, C. M. J.
  • Gil Sanchez, Luis Alfonso
  • Martin Garcia, Juan Antonio
Item Type: Presentation at Congress or Conference (Poster)
Event Title: LIFE+ ELMIAS Ash and Elm, and IUFRO WP 7.02.01 Root and Stem Rots Conference (LIFE-IUFRO)
Event Dates: 26 Agosto - 1 Septiembre
Event Location: Uppsala
Title of Book: PROGRAM & BOOK OF ABSTRACTS
Date: 2018
Subjects:
Freetext Keywords: Hongos endofíticos, Ulmus minor.
Faculty: E.T.S.I. Montes, Forestal y del Medio Natural (UPM)
Department: Sistemas y Recursos Naturales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Dutch elm disease (DED) is caused by the fungus Ophiostoma novo-ulmi and is one of the most devastating forest diseases of the last century. However, there are some elm genotypes able to tolerate this disease. Although some mechanisms involved in the tolerance have been already characterized, the molecular basis of the tolerance is still unknown. In addition, the role of the tree microbiome in elm tolerance to DED is poorly known. In this work, we aimed to observe the molecular responses of tolerant and susceptible Ulmus minor genotypes after the inoculation of either the pathogen or one endophytic fungus of the genus Rhodotorula which was found to be more abundantly present in tolerant than susceptible genotypes. We worked with 1 month-old plantlets grown in vitro. The roots were submerged in a solution of spores of each fungus and later the plants were grown for a week preserving the sterility. The expression of 7 genes related to local defense responses was analyzed in the roots. Both fungi produced a strong overexpression of phenylalanine ammonia lyase (PAL) and pathogenesis-related protein 4 (PR4) genes. However the pathogen induced a higher response than the endophyte. In a second experiment, we explored the ability of the endophyte to induce resistance in the elm against O. novo-ulmi. Then, a new treatment included the inoculation with Rhodotorula prior to the pathogen inoculation. In comparison with the single inoculation of O. novo-ulmi, the double inoculation showed a reduction of the PAL overexpression in tolerant genotypes, while no effect or increased expression was observed in the susceptible ones. These results suggest a relationship between the tolerance to DED and the association of certain endophytes to some elm genotypes.

More information

Item ID: 55533
DC Identifier: http://oa.upm.es/55533/
OAI Identifier: oai:oa.upm.es:55533
Deposited by: Memoria Investigacion
Deposited on: 25 Jun 2019 09:21
Last Modified: 25 Jun 2019 09:21
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