bZIP and DOF transcription factors in the regulation of gene expression in barley endosperm

Carbonero Zalduegui, Pilar and Vicente Carbajosa, Jesus and Mena, Montaña and Oñate Sanchez, Luis and Lara, Pilar and Diaz Rodriguez, Isabel (1999). bZIP and DOF transcription factors in the regulation of gene expression in barley endosperm. In: "Sixth International Workshop on Seeds", Enero, 1999, Mérida, México. p. 15.

Description

Title: bZIP and DOF transcription factors in the regulation of gene expression in barley endosperm
Author/s:
  • Carbonero Zalduegui, Pilar
  • Vicente Carbajosa, Jesus
  • Mena, Montaña
  • Oñate Sanchez, Luis
  • Lara, Pilar
  • Diaz Rodriguez, Isabel
Item Type: Presentation at Congress or Conference (Article)
Event Title: Sixth International Workshop on Seeds
Event Dates: Enero, 1999
Event Location: Mérida, México
Title of Book: Seed Biology: Advances and Applications: Proceedings of the Sixth International Workshop on Seeds
Date: January 1999
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The protein quality of cereal grain is determined by the quantity and composition of the prolamin storage proteins (hordeins in barley) that are specifically synthesized in the endosperm and that are regulated at the transcriptional level. Most prolamin gene promoters contain a bipartite sequence motif in the -300 region that becomes occupied early in endosperm development by nuclear trans -acting factors. cDNAs have been characterized from barley endosperm encoding DNA-binding proteins with affinity towards the two halves of the -300 motif: 5'TGTAAAG-3' prolamin box; PB) and 5'-GTGAGTCAT-3'(GCN4-like; GLM), respectively. The barley PB binding factor is a zinc finger of the DOF class while the GLM binding factors belong to the bZIP class of transcription factors. Experiments in vivo and in vitro are described that strongly implicate these factors as important activators of hordein gene expression and suggest their evolutionary conservation among small grain cereals.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainPB97-0561UnspecifiedPilar Carbonero ZaldueguiRegulación genética en semillas de cereales: caracterización molecular de factores transcripcionales tipo BZIP y DOF

More information

Item ID: 56670
DC Identifier: http://oa.upm.es/56670/
OAI Identifier: oai:oa.upm.es:56670
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 04 Oct 2019 09:02
Last Modified: 04 Oct 2019 09:02
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