GAMYB and BPBF Transcriptional Factors in the Control of Gene Expression During Development of Barley Endosperm

Diaz Rodriguez, Isabel and Vicente Carbajosa, Jesus and Abraham, Zamira and Martinez Muñoz, Manuel and Isabel La Moneda, Inés and Carbonero Zalduegui, Pilar (2003). GAMYB and BPBF Transcriptional Factors in the Control of Gene Expression During Development of Barley Endosperm. In: "Seventh International Workshop on Seeds", Mayo, 2002, Salamanca, España. p. 8.

Description

Title: GAMYB and BPBF Transcriptional Factors in the Control of Gene Expression During Development of Barley Endosperm
Author/s:
  • Diaz Rodriguez, Isabel
  • Vicente Carbajosa, Jesus
  • Abraham, Zamira
  • Martinez Muñoz, Manuel
  • Isabel La Moneda, Inés
  • Carbonero Zalduegui, Pilar
Item Type: Presentation at Congress or Conference (Article)
Event Title: Seventh International Workshop on Seeds
Event Dates: Mayo, 2002
Event Location: Salamanca, España
Title of Book: The Biology of Seeds: Recent Research Advances: Proceedings of the Seventh International Workshop on Seeds
Date: October 2003
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

During cereal seed development, the starchy endosperm cells accumulate proteins, mainly prolamines, as well as several antimicrobial peptides and inhibitors of heterologous hydrolases implicated in plant defence (Shewry et al ., 1995; Carbonero et al ., 1999). The genes encoding the storage proteins are coordinately expressed in the developing endosperm where they are under spatial and temporal transcriptional control, involving cis-acting motifs in their promoters and trans-acting transcriptional factors (TFs). A number of consensus sequences in gene promoters have been shown to be involved in conferring endosperm specificity in cereals (Albani et al ., 1997; Marzabal et al ., 1998; Mena et al ., 1998; Vicente-Carbajosa et al ., 1998; Oñate et al ., 1999; Carbonero et al ., 2000; Wu et al ., 2000; Díaz et al ., 2002). A conserved cis-acting motif found in most storage protein gene promoters of seeds is the endosperm-box, a bipartite motif located ~300 bp upstream of the translation initiation codon that contains two distinct binding sites: the GCN4-like motif (GLM) motif (5´-ATGAG/CTCAT-3´) that resembles the yeast GCN4 motif and the prolamine box (PB: 5´-TGTAAAG-3´). There is a third motif (5´-AACA/TA-3´), which plays a role in the expression of genes encoding § glutelins in rice (Suzuki et al ., 1998). These three types of motifs are the target sites of different TFs. In barley, the GLM is recognized by bZIP proteins of the Opaque2 family (BLZ1 and BLZ2) that through dimer formation, as either homo- or heterodimers (Vicente-Carbajosa et al ., 1998; Oñate et al ., 1999), trans-activate the expression of endosperm-specific genes such as the Hor2 and Itr1 genes encoding the B-hordeins and a trypsin inhibitor, respectively. The prolamine box is recognized by a TF of the DOF class, DNA binding with One Finger (BPBF), that activates transcription of a native Hor2 promoter in co-bombarded barley endosperms through binding to the prolamine box (PB) We describe here how the 5´-AACA/TA-3´ motif interacts with a TF of the MYB class, which was previously shown to have a role in the activation of gibberellin (GA)-induced genes upon germination in barley aleurone cells (Gubler et al ., 1995, 1999).

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBCM2000-1483UnspecifiedPilar Carbonero ZaldueguiGenes reguladores del desarrollo de la germinación de semillas de cebada y Arabidopsis
Madrid Regional Government07G/0015/2000UnspecifiedUnspecifiedUnspecified

More information

Item ID: 56869
DC Identifier: http://oa.upm.es/56869/
OAI Identifier: oai:oa.upm.es:56869
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 14 Oct 2019 12:36
Last Modified: 14 Oct 2019 12:36
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