Análisis genético de la calidad panadera en una colección española de variedades locales de trigo blando

Faci Gómez, Isabel (2019). Análisis genético de la calidad panadera en una colección española de variedades locales de trigo blando. Proyecto Fin de Carrera / Trabajo Fin de Grado, E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM), Madrid.

Description

Title: Análisis genético de la calidad panadera en una colección española de variedades locales de trigo blando
Author/s:
  • Faci Gómez, Isabel
Contributor/s:
  • Pascual Bañuls, Laura
Item Type: Final Project
Degree: Grado en Biotecnología
Date: June 2019
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Wheat occupies a central place in human nutrition providing 20% of the daily protein and food calories. Current cultivated wheat has originated from natural hybridization events between different species. Roughly 90 to 95% of the wheat produced in the world is common or bread wheat (Triticum aestivum L.; 2n= 6x= 42, 17Gb, AABBDD genomes). So, any advances related with breeding in this species will have a major worldwide impact. Wheat breeding has mainly been focused on yield improvement, however lately an interest in flour quality has risen. In order to conduct successful breeding for any trait, it is necessary to start from a wide range of variation, and ideally to have deep knowledge about its genetic control. When compared to other germplasm collections, the Spanish wheat landraces conserved at National Plant Genetic Resources Center (CRF-INIA) have shown the great variability of these materials. In the present work we have addressed the characterization of a representative set from the Spanish bread wheat landraces (189 accessions). We have explored quality by focusing on protein content, hardness and SSDS volume. We also have explored the association between those traits and glutamine synthetase genes that have a key role in plant metabolism. Moreover, we have extended these analyses to cover the full wheat genome conducting a Genome Wide Association Analysis, based on a set of 4282 markers which had been previously genotyped in these same accessions. Our analysis allowed us to describe the wide range of variation in these accessions and to identify some that might be useful for specific breeding objectives. We have also compared the values that were obtained in different years and established that hardness, followed by SSDS volume, was quite stable in different environments, while protein content was more variable. Regarding the genetic control, we have been able to establish a tight link between glutamine synthetase genes and protein content, not only when analyzing those genes individually, but also at genomic level. With respect to hardness we fail to detect associations with 5D chromosome, a well-known region related with this trait. However, we have detected associations in more than 10 regions that were quite stable under different environments. Finally, regarding to SSDS volume, related with flour strength, we have observed associations with the regions that include the main glutenin genes as expected, but also some new associations in 2B, 3D, 5A, 5D and 6D. When we consider our full set of analysis, we have detected two main regions in 1B and 5A where a wide number of associations were found. Those will be interesting targets for quality breeding.

More information

Item ID: 56934
DC Identifier: http://oa.upm.es/56934/
OAI Identifier: oai:oa.upm.es:56934
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 18 Oct 2019 11:35
Last Modified: 18 Oct 2019 11:35
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