Expresión de IL-15 en pacientes con síndrome de Stevens-Johnson/necrolisis epidérmica tóxica

Martínez del Río, Javier (2019). Expresión de IL-15 en pacientes con síndrome de Stevens-Johnson/necrolisis epidérmica tóxica. Proyecto Fin de Carrera / Trabajo Fin de Grado, E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM), Madrid.

Description

Title: Expresión de IL-15 en pacientes con síndrome de Stevens-Johnson/necrolisis epidérmica tóxica
Author/s:
  • Martínez del Río, Javier
Contributor/s:
  • Bellón Heredia, Teresa
  • Martínez Muñoz, Manuel
Item Type: Final Project
Degree: Grado en Biotecnología
Date: June 2019
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The Stevens-Johnson syndrome (SJS) and the toxic epidermal necrolysis (TEN) are two rare diseases; however, they have significant morbidity and mortality. These are type IV hypersensitivity reactions in which T lymphocytes direct the immune response. In most cases these cells arise in response to a drug, but there are also other causative agents such as infectious pathogens. The most relevant clinical characteristic of these conditions is the appearance of lesions on the skin. In fact, SJS and TEN differ according to the proportion of affected body skin, with TEN being the most serious one. An important consequence of these lesions is the detachment of the outermost layer of the skin due to the apoptosis of keratinocytes. The mechanisms inducing apoptosis are not known in detail. It is known that different immune cells and soluble mediators intervene. Among the latter, it has recently been discovered that the serum levels of interleukin-15 (IL-15) correlate with the level of severity of the disease, with IL-15 playing an important role in immunopathogenesis. In this project, the expression of IL15 and the alpha subunit of its receptor (IL15RA) have been evaluated in SJS/TEN patients and the expression levels compared with those in healthy control subjects. In this way, the differences found have been examined and we have studied the relationship of IL15 expression with the severity of the illness. Peripheral blood samples and skin biopsies were obtained, ribonucleic acid (RNA) has been extracted and the expression of both genes has been evaluated by quantitative Polymerase Chain Reaction (qPCR). In skin samples, it has been found that there is a slightly higher expression of IL15 in patients; although not statistically significant, which is accompanied by an exclusive expression of IL15RA, which has not been observed in healthy control skin. This has allowed us to conclude that not only IL-15 can play an important role in the cytotoxic activity against keratinocytes; but the expression of IL15RA can also be decisive. On the other hand, no statistically significant differences were found in the expression of IL15 in peripheral blood mononuclear cells (PBMCs) compared to healthy control subjects, whereas the expression of IL15RA has not been detected. These results suggest that the expression of IL15 in PBMCs might have little relevance in the etiopathogenesis of the disease. This study has also found a relationship between the levels of expression of IL15 in skin and the severity of SJS/TEN. In conclusion, this project has found evidence of the relation of IL-15 with the etiopathogenesis of the disease as it is related to the expression of IL15RA in affected skin. However, research in this topic is still necessary to continue evaluating in more detail the role of IL-15 and IL-15R in SJS/TEN and be able to find more knowledge about the pathological mechanism of this disease, which remains unknown in detail. All this effort, without a doubt, can help in the future to find suitable tools for early diagnosis and curative treatments in a disease that reaches a mortality of 30%.

More information

Item ID: 57047
DC Identifier: http://oa.upm.es/57047/
OAI Identifier: oai:oa.upm.es:57047
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 24 Oct 2019 08:01
Last Modified: 24 Oct 2019 08:01
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