Mejora de la producción fotobiológica de hidrógeno por la bacteria Rhodobacter capsulatus

San Isidro Martín, Elisa (2019). Mejora de la producción fotobiológica de hidrógeno por la bacteria Rhodobacter capsulatus. Proyecto Fin de Carrera / Trabajo Fin de Grado, E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM), Madrid.

Description

Title: Mejora de la producción fotobiológica de hidrógeno por la bacteria Rhodobacter capsulatus
Author/s:
  • San Isidro Martín, Elisa
Contributor/s:
  • Barahona Martín, Emma
Item Type: Final Project
Degree: Grado en Biotecnología
Date: June 2019
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

In these last years, the energy demand has increased. The predominant primary energies are non-renewable and polluting. This is driving to the depletion and destruction of the planet in a close future. Against this background, different investigations have focused on trying to find alternative energies. Among them, investigations have greatly opted for hydrogen (H2); specifically in its biologic production. This research is centered in the analysis and optimization of H2 production by photofermentation in purple non-sulfur bacteria (PNS), in particular, in the nitrogen-fixing bacterium Rhodobacter capsulatus. For this purpose, we have used overproducer mutant strains, obtained by random mutagenesis through irradiation with ultraviolet light. The capacity of H2 production was determined in closed cultures, based on the amount of H2 produced and the nitrogenase activity within each of them. To enhance H2 generation and reduce productions costs, these strains were grown with different carbon sources: glucose, sucrose and sodium acetate. Another strategy to improve it consists of the use of continuous cultivations to avoid the depletion of nutrients and, therefore, the termination of the production. In this study, it is proved that a continuous and increased production of H2 by R. capsulatus can be implemented. Moreover, the use of continuous cultivation allowed us to improve the production 3,374% compared to the control strain.

More information

Item ID: 57131
DC Identifier: http://oa.upm.es/57131/
OAI Identifier: oai:oa.upm.es:57131
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 30 Oct 2019 09:51
Last Modified: 30 Oct 2019 09:51
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