Recognition motifs rather than phylogenetic origin influence the ability of targeting peptides to import nuclear-encoded recombinant proteins into rice mitochondria

Baysal, Can and Pérez González, Ana and Eseverri, Álvaro and Jiang, Xi and Medina, Vicente and Caro Bernat, Elena and Rubio Herrero, Luis Manuel and Christou, Paul and Zhu, Chansfu (2020). Recognition motifs rather than phylogenetic origin influence the ability of targeting peptides to import nuclear-encoded recombinant proteins into rice mitochondria. "Transgenic Research", v. 29 (n. 2); pp. 37-52. ISSN 0962-8819. https://doi.org/10.1007/s11248-019-00176-9.

Description

Title: Recognition motifs rather than phylogenetic origin influence the ability of targeting peptides to import nuclear-encoded recombinant proteins into rice mitochondria
Author/s:
  • Baysal, Can
  • Pérez González, Ana
  • Eseverri, Álvaro
  • Jiang, Xi
  • Medina, Vicente
  • Caro Bernat, Elena
  • Rubio Herrero, Luis Manuel
  • Christou, Paul
  • Zhu, Chansfu
Item Type: Article
Título de Revista/Publicación: Transgenic Research
Date: April 2020
ISSN: 0962-8819
Volume: 29
Subjects:
Freetext Keywords: Subcellular targeting; Mitochondrial pre-sequence; Mitochondrial protein; Protein sorting; Green fluorescent protein
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Mitochondria fulfil essential functions in respiration and metabolism as well as regulating stress responses and apoptosis. Most native mitochondrial proteins are encoded by nuclear genes and are imported into mitochondria via one of several receptors that recognize N-terminal signal peptides. The targeting of recombinant proteins to mitochondria therefore requires the presence of an appropriate N-terminal peptide, but little is known about mitochondrial import in monocotyledonous plants such as rice (Oryza sativa). To gain insight into this phenomenon, we targeted nuclear-encoded enhanced green fluorescent protein (eGFP) to rice mitochondria using six mitochondrial pre-sequences with diverse phylogenetic origins, and investigated their effectiveness by immunoblot analysis as well as confocal and electron microscopy. We found that the ATPA and COX4 (Saccharomyces cerevisiae), SU9 (Neurospora crassa), pFA (Arabidopsis thaliana) and OsSCSb (Oryza sativa) peptides successfully directed most of the eGFP to the mitochondria, whereas the MTS2 peptide (Nicotiana plumbaginifolia) showed little or no evidence of targeting ability even though it is a native plant sequence. Our data therefore indicate that the presence of particular recognition motifs may be required for mitochondrial targeting, whereas the phylogenetic origin of the pre-sequences probably does not play a key role in the success of mitochondrial targeting in dedifferentiated rice callus and plants.

More information

Item ID: 57186
DC Identifier: http://oa.upm.es/57186/
OAI Identifier: oai:oa.upm.es:57186
DOI: 10.1007/s11248-019-00176-9
Official URL: https://link.springer.com/article/10.1007%2Fs11248-019-00176-9
Deposited by: Memoria Investigacion
Deposited on: 27 Mar 2020 11:50
Last Modified: 27 Mar 2020 11:50
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