Identificación de biomarcadores en pacientes diabéticos con fragilidad ósea mediante metabolómica

González Marín, Alejandro (2019). Identificación de biomarcadores en pacientes diabéticos con fragilidad ósea mediante metabolómica. Proyecto Fin de Carrera / Trabajo Fin de Grado, E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM), Madrid.

Description

Title: Identificación de biomarcadores en pacientes diabéticos con fragilidad ósea mediante metabolómica
Author/s:
  • González Marín, Alejandro
Contributor/s:
  • Villaseñor Solís, Alma Cristina
  • Díaz Perales, Araceli
Item Type: Final Project
Degree: Grado en Biotecnología
Date: June 2019
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img] PDF - Users in campus UPM only - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (3MB)

Abstract

Diabetes Mellitus (DM) is a disease with a high worldwide prevalence, and it will continue increasing in the future. Moreover, almost 90% of DM patients suffer diabetes mellitus type 2 (DM2). This disease might lead to several comorbidities, such as bone fragility. Traditionally, bone fragility is diagnosed by the measurement of bone mineral density (BMD). However, in the case of DM2 this is not a good predictor as the BMD in these patients is normal or even higher. Therefore, there is an urgent necessity of accurate biomarkers that could diagnose bone fragility in DM2 patients. Metabolomics using a non-targeted analysis approach allows the analysis of a high number of chemical signals from a biological sample, from which characteristic biomarkers of the studied samples can be acquired through a differential analysis. With this approach, molecular mechanisms for how DM2 affects the bone can be explored, leading to a better understanding of the pathology. In this clinical study, 81 patients were enrolled and classified as diabetics (D), diabetics with bone fragility (D-Frag) or age-matched subjects (Controls). Plasma samples were extracted and analyzed using liquid chromatography coupled to mass spectrometry (LC-MS). After data treatment, 990 metabolites were obtained in each sample with high quality. From the groups, the comparison of the diabetic men vs diabetic men with bone fragility was selected as a key model to understand the pathology. After statistical analysis 80 significant metabolites were obtained, all of which were isolated and fragmented through tandem mass spectrometry (MS/MS). Up to now, hexanoylcarnitine, octanoylcarnitine and decanoylcarnitine were found to be increased in D-Frag. Moreover, cholesterol, lysophophocholine 20:4 and lysophosphoethanolamine 16:0 were found decreased in D-Frag. Further studies are needed in order to confirm their identity as biomarkers for the disease and how they are implied in it. This study demonstrates the capabilities of metabolomics in exploring complex diseases in humans. Further analyses are needed to clarify our results.

More information

Item ID: 57503
DC Identifier: http://oa.upm.es/57503/
OAI Identifier: oai:oa.upm.es:57503
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 13 Dec 2019 10:58
Last Modified: 13 Dec 2019 10:58
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM