Caracterización del Sistema del Complemento en la Retinosis Pigmentaria

Martínez Olondo, Pilar (2019). Caracterización del Sistema del Complemento en la Retinosis Pigmentaria. Proyecto Fin de Carrera / Trabajo Fin de Grado, E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM), Madrid.

Description

Title: Caracterización del Sistema del Complemento en la Retinosis Pigmentaria
Author/s:
  • Martínez Olondo, Pilar
Contributor/s:
  • Hernández Sánchez, Catalina
  • González-Melendi, Pablo
Item Type: Final Project
Degree: Grado en Biotecnología
Date: June 2019
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img] PDF - Users in campus UPM only - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (1MB)

Abstract

Retinitis Pigmentosa (RP) represents a heterogeneous group of inherited retinal dystrophies that courses with progressive photoreceptor degeneration and death, leading to visual loss and blindness. This disease is caused by mutations (more than 50) predominantly in photoreceptor-specific genes. Photoreceptor loss progresses with retinal remodelling, microglial recruitment, reactive gliosis and inflammation. Treatment for RP patients is not currently available. The innate immune response is the first line of defence of the organism. The complement system is an effector of this response and plays an essential role in eliminating and clearance of infected and damaged cells. However, it has been shown that elements of the complement system are involved in neurodegenerative disorders contributing to inflammation and synapse loss. In previous studies of our group, we have observed overexpression of complement components in retinas of a mouse model of Retinitis Pigmentosa (rd10). C1q, a component of the classical component pathway, was highly elevated since early stages of the disease. In this work, we have characterized the presence and distribution of C1qa throughout the retina in wild type and rd10 mice. The results showed that C1qa levels were increased in microglial cells in the rd10 retinas respect to the wild type retinas. Besides the microglial expression, we found C1qa deposits in the outer plexiform layer of the retina associated to the synaptic triads formed by photoreceptors and horizontal and bipolar cells. Furthermore, rd10 retinas displayed higher accumulation of C1qa in the synaptic triads than in wild type retinas. Intravitreal injection of a blocking-C1q anti-serum in rd10 mice preserved the visual function compared to control mice, as shown by increased electroretinogram (ERG) a- and b-wave amplitudes. Together, these results indicate that the complement system, particularly C1q, may be involved in the retinal degeneration associated to RP, and that interference with the complement system is a potential therapeutic strategy for the treatment of the disease.

More information

Item ID: 57527
DC Identifier: http://oa.upm.es/57527/
OAI Identifier: oai:oa.upm.es:57527
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 16 Dec 2019 13:47
Last Modified: 16 Dec 2019 13:47
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM