Criopreservación de células mediante Limbo y comparación con la criopreservación de rutina

Mesa Sánchez, Rocío (2019). Criopreservación de células mediante Limbo y comparación con la criopreservación de rutina. Proyecto Fin de Carrera / Trabajo Fin de Grado, E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM), Madrid.

Description

Title: Criopreservación de células mediante Limbo y comparación con la criopreservación de rutina
Author/s:
  • Mesa Sánchez, Rocío
Contributor/s:
  • García Arranz, Mariano
  • Carrió González, Elena
Item Type: Final Project
Degree: Grado en Biotecnología
Date: June 2019
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Mesenchymal stem cells (MSC) have the ability to differenciate themself into differents cell types, such as osteocytes, myocytes, adypocites and chondrocytes. Moreover, these cells secrete citokines and grow factors which promote tissue repair processes and modulate the inmmune response. These properties give MSC some advantages for applications in cellular therapy. The adipose tissue is one important source of MSC, being these cells known as adipose derived stem cells (ASCs) The aim of this research is to develop a method to freeze cells with less toxicity than the routin method that is commonly use for cellular therapies. The new cryopreservation method proposed here is based on a device called Limbo and its benefit consist in diluting DymethilSulfoxide (DMSO) in the thawing with the aim of being safer for their application in humans. In addition, it is faster and controllable than the standard method since cells thawing by the Limbo method don´t need to be grown in culture. Cells could be directly inyected into a patient. To study its efficiency, this method was compared to the standard method. For that, studies into chromosomes mutations, cell-surface receptors, differenciation ability, cell viability, growth rate and citokines expression were carried out. Results show ASCs characteristics don´t change between both of methods of crypreservation. Limbo method gets a higher concentration of growth factors and anti-inflammation cytokines and lower pro-inflammation cytokines. Furthermore, adipocytes differenciation availability of ASCs increased in cells treated by the Limbo method. I conclude that Limbo method provide advantages to cells for their application in cellular therapy, although the method should be improved in futher research.

More information

Item ID: 57537
DC Identifier: http://oa.upm.es/57537/
OAI Identifier: oai:oa.upm.es:57537
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 17 Dec 2019 13:31
Last Modified: 17 Dec 2019 13:31
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